Association of heavy metals with metallothionein and other proteins in hepatic cytosol of marine mammals and seabirds
Association of heavy metals with metallothionein and other proteins in hepatic cytosol of marine mammals and seabirds
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DOI：
10.1897/03-456
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发表时间：
2004-08-01
影响因子：
4.1
通讯作者：
Tanabe, S
中科院分区：
文献类型：
--
作者：
Ikemoto, T;Kunito, T;Tanabe, S
测定了北方海狗（Callorhinus ursinus）、黑足信天翁（Diomedea nigripes）和鼠海豚（Phocoenoides dalli）肝细胞溶胶中Cu、Zn、Cd、Ag、Hg和Se的分布。Cu、Zn、Cd优先富集在金属硫蛋白（MT）组分中，且MT组分中Cu、Zn、Cd的含量随肝细胞液中Cu、Zn、Cd含量的增加而增加。银结合的高分子量物质（HMWS）和MT在肝细胞溶胶中的所有三个物种，而银在细胞溶胶中的分布是不同的三个物种。在北方海狗中，Ag主要与MT结合，而在达尔鼠海豚中，Ag主要与HMWS结合。与此相反，银几乎均匀分布在HMWS和MT的黑脚信天翁。高分子量物质中汞含量、高分子量物质和低分子量物质中硒含量均随肝细胞液中汞含量的增加而增加。细胞质中高分子量（HMW）组分的硒和汞含量之间呈显著正相关。高浓度汞的样品中，高分子量组分中汞和硒的摩尔比接近1，这意味着汞硒络合物与HMWS结合。通过高效液相色谱/电感耦合等离子体质谱（HPLC/ICP-MS）的肝细胞溶胶中的金属分析表明，MT的多种异构体存在于肝细胞溶胶的三个物种和肝细胞溶胶中的金属配置文件是不同的物种。据我们所知，这是第一次报告。海洋哺乳动物和海鸟肝细胞溶胶中Ag与HMWS和NIT的关联。首次研究了汞和硒在高营养海洋动物肝细胞溶胶中的分布及其相互作用。
Distribution of Cu, Zn, Cd, Ag, Hg, and Se were determined in hepatocytosol of northern fur seals (Callorhinus ursinus), black-footed albatrosses (Diomedea nigripes), and Dall's porpoises (Phocoenoides dalli). Copper, Zn, and Cd were accumulated preferentially in metallothionein (MT) fraction and their contents in MT fraction increased with the amounts in the hepatocytosol. Silver was bound to both high-molecular-weight substances (HMWS) and MT in the hepatocytosol for all three species, whereas the distribution of Ag in the cytosol was different among the three species. In northern fur seals, Ag mainly was bound to MT, whereas it mainly was associated with HMWS in Dall's porpoises. In contrast, Ag was distributed almost equally in both HMWS and MT for black-footed albatrosses. Mercury content in HMWS and Se content in HMWS and low-molecular-weight substances (LMWS) increased with their contents in hepatocytosol for all the three species. A significant positive correlation was found between Se and Hg contents in high-molecular weight (HMW) fraction in cytosol. The molar ratio of Hg and Se was close to unity in HMW fraction of the specimens with high Hg concentration in cytosol, implying that the Hg-Se complex was bound to the HMWS. Analysis of metals in the hepatocytosol by high-performance liquid chromatography/inductively coupled plasma-mass spectrometry (HPLC/ICP-MS) suggests that multiple isoforms of MT are present in hepatocytosol of the three species and that the metal profiles in hepatocytosols are different among the species. To our knowledge, this is the first report on the. association of Ag with HMWS and NIT in hepatocytosol of marine mammals and seabirds. Also, distribution and interaction of Hg and Se were investigated for the first time in hepatocytosol of the higher trophic marine animals.