Microlens systems for fluorescence detection in chemical microsystems
Microlens systems for fluorescence detection in chemical microsystems
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DOI：
10.1117/1.1359522
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发表时间：
2001-05
影响因子：
1.3
通讯作者：
J.-C. Roulet;Reinhard Vo¨lkel;H. Herzig;E. Verpoorte;N. Rooij;Rene´ Da¨ndliker
中科院分区：
文献类型：
--
作者：
J.-C. Roulet;Reinhard Vo¨lkel;H. Herzig;E. Verpoorte;N. Rooij;Rene´ Da¨ndliker
研究了基于折射微透镜的微光学系统。这些系统集成在化学芯片上。它们将激发光束聚焦到检测体积（微升甚至亚微升规模）并收集荧光分子发出的光。必须通过空间和光谱过滤仔细地将荧光与激发分开。本文介绍了三种照明系统的光线追踪模拟、制造和测量。测量结果表明，微制造透镜和光阑的巧妙放置和组合可以增加激发光和荧光之间的分离。此外，我们使用其中一种照明系统成功检测了 100 毫米宽、50 毫米深的微通道（激发体积为 250 pL）中的 20 nM Cy5™（Amersham Life Science Ltd.）溶液。具有微光学系统的微化学芯片的厚度小于2mm。 © 2001 年光电仪器工程师协会。 （DOI：10.1117/1.1359522）
Micro-optical systems based on refractive microlenses are in- vestigated. These systems are integrated on a chemical chip. They focus an excitation beam into the detection volume (microliter or even submi- croliter scale) and collect the emitted light from fluorescent molecules. The fluorescence must be carefully separated by spatial and spectral filtering from the excitation. This paper presents the ray tracing simula- tion, fabrication, and measurement of three illumination systems. The measurements show that an adroit placement and combination of micro- fabricated lenses and stops can increase the separation between the excitation light and the fluorescence light. Moreover we present the suc- cessful detection of a 20 nM Cy5™ (Amersham Life Science Ltd.) solu- tion in a 100-mm-wide and 50-mm-deep microchannel (excitation volume '250 pL) using one of these illumination systems. The microchemical chip with the micro-optical system has a thickness of less than 2 mm. © 2001 Society of Photo-Optical Instrumentation Engineers. (DOI: 10.1117/1.1359522)