Expansion of the receptor-like kinase/Pelle gene family and receptor-like proteins in Arabidopsis
Expansion of the receptor-like kinase/Pelle gene family and receptor-like proteins in Arabidopsis
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DOI：
10.1104/pp.103.021964
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发表时间：
2003-06-01
期刊：
影响因子：
7.4
通讯作者：
Bleecker, AB
中科院分区：
文献类型：
--
作者：
Shiu, SH;Bleecker, AB
受体样激酶 (RLK) 是一个跨膜蛋白家族，具有多功能 N 端胞外结构域和 C 端胞内激酶。它们控制植物中广泛的生理反应，属于拟南芥基因组中最大的基因家族之一，拥有 600 多个成员。有趣的是，该基因家族占拟南芥中所有激酶的 60%，并且几乎占拟南芥中所有跨膜激酶。四种真菌、六种后生动物和两种疟原虫的分析。基因组表明该家族在除真菌基因组之外的所有基因组中都有代表，这表明该家族的起源很古老，最近仅在植物谱系中有所扩展。 RLK/Pelle 家族可根据三个独立标准分为几个亚家族：基于激酶结构域序列的系统发育、胞外结构域身份以及内含子位置和阶段。在拟南芥基因组中还发现了大量类似于 RLK 胞外域的受体样蛋白 (RLP)。然而，并非所有 RLK 亚科都有相应的 RLP。几个 RLK/Pelle 亚科经历了差异扩张。超过 33% 的 RLK/Pelle 成员存在于串联簇中，远高于基因组平均水平。此外，470个RLK/Pelle家族成员位于拟南芥基因组的片段重复区域内，其中268个在相应区域有近亲。因此，串联重复和片段/全基因组重复代表了拟南芥中 RLK/Pelle 家族扩张的两种主要机制。
Receptor-like kinases (RLKs) are a family of transmembrane proteins with versatile N-terminal extracellular domains and C-terminal intracellular kinases. They control a wide range of physiological responses in plants and belong to one of the largest gene families in the Arabidopsis genome with more than 600 members. Interestingly, this gene family constitutes 60% of all kinases in Arabidopsis and accounts for nearly all transmembrane kinases in Arabidopsis. Analysis of four fungal, six metazoan, and two Plasmodium sp. genomes indicates that the family was represented in all but fungal genomes, indicating an ancient origin for the family with a more recent expansion only in the plant lineages. The RLK/Pelle family can be divided into several subfamilies based on three independent criteria: the phylogeny based on kinase domain sequences, the extracellular domain identities, and intron locations and phases. A large number of receptor-like proteins (RLPs) resembling the extracellular domains of RLKs are also found in the Arabidopsis genome. However, not all RLK subfamilies have corresponding RLPs. Several RLK/Pelle subfamilies have undergone differential expansions. More than 33% of the RLK/Pelle members are found in tandem clusters, substantially higher than the genome average. In addition, 470 of the RLK/Pelle family members are located within the segmentally duplicated regions in the Arabidopsis genome and 268 of them have a close relative in the corresponding regions. Therefore, tandem duplications and segmental/whole-genome duplications represent two of the major mechanisms for the expansion of the RLK/Pelle family in Arabidopsis.