Long and short term effects of plasma treatment on meristematic plant cells
Long and short term effects of plasma treatment on meristematic plant cells
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DOI：
10.1063/1.4880360
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发表时间：
2014-05-26
影响因子：
4
通讯作者：
Petrovic, Z. Lj
中科院分区：
文献类型：
--
作者：
Puac, N.;Zivkovic, S.;Petrovic, Z. Lj
本文将介绍等离子体处理胡萝卜分生组织细胞的结果。在所有处理中，血浆针作为非平衡等离子体的大气压/气体成分源。在血浆治疗后立即和治疗后两周测定抗氧化酶、超氧化物歧化酶和过氧化氢酶的活性。血浆处理后，样品中超氧化物歧化酶活性立即增加。另一方面，在血浆处理两周后，过氧化氢酶活性在处理过的样品中要高得多。这些结果表明，有直接证据表明，两种活性氧H2O2和O-2（-）在细胞中触发信号转导，即使在愈伤组织生长过程中也会引起酶活性和短期和长期的影响，并在两周内将信息传递给新生细胞。(C) 2014 AIP出版有限责任公司
In this paper, we will present results of plasma treatments of meristematic cells of Daucus carota. Plasma needle was used as an atmospheric pressure/gas composition source of non-equilibrium plasma in all treatments. Activity of antioxidant enzymes superoxide dismutase and catalase was measured immediately after plasma treatment and after two weeks following the treatment. Superoxide dismutase activity was increased in samples immediately after the plasma treatment. On the other hand, catalase activity was much higher in treated samples when measured two weeks after plasma treatment. These results show that there is a direct proof of the triggering of signal transduction in the cells by two reactive oxygen species H2O2 and O-2(-), causing enzyme activity and short and long term effects even during the growth of calli, where the information is passed to newborn cells over the period of two weeks. (C) 2014 AIP Publishing LLC.