Overproduction of Native and Click-able Colanic Acid Slime from Engineered Escherichia coli.
Overproduction of Native and Click-able Colanic Acid Slime from Engineered Escherichia coli.
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DOI：
10.1021/jacsau.2c00583
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发表时间：
2023-02-27
期刊：
影响因子：
8
通讯作者：
Wallace, Stephen
中科院分区：
文献类型：
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作者：
Sadler, Joanna C;Brewster, Richard C;Kjeldsen, Annemette;Gonzalez, Alba F;Nirkko, Jessica S;Varzandeh, Simon;Wallace, Stephen
细菌胞外多糖的基础生物学和应用越来越受到人们的关注。然而，目前合成生物学的努力，以生产的主要成分，大肠杆菌属粘液，colanic酸，及其功能衍生物已受到限制。在此，我们报告了大肠杆菌JM 109的工程菌株中从d-葡萄糖过量生产柯拉酸（高达1.32 g/L）。此外，我们报告说，化学合成的L-岩藻糖类似物含有叠氮化物基序可以代谢纳入粘液层通过异源岩藻糖抢救途径从拟杆菌属和点击反应中使用的附加有机货物的细胞表面。这种分子工程生物聚合物有潜力作为一种新的工具，用于化学，生物和材料研究。
The fundamental biology and application of bacterial exopolysaccharides is gaining increasing attention. However, current synthetic biology efforts to produce the major component of Escherichia sp. slime, colanic acid, and functional derivatives thereof have been limited. Herein, we report the overproduction of colanic acid (up to 1.32 g/L) from d-glucose in an engineered strain of Escherichia coli JM109. Furthermore, we report that chemically synthesized l-fucose analogues containing an azide motif can be metabolically incorporated into the slime layer via a heterologous fucose salvage pathway from Bacteroides sp. and used in a click reaction to attach an organic cargo to the cell surface. This molecular-engineered biopolymer has potential as a new tool for use in chemical, biological, and materials research.