Effects of Curcumin Nanoparticles in Isoproterenol-Induced Myocardial Infarction
Effects of Curcumin Nanoparticles in Isoproterenol-Induced Myocardial Infarction
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DOI：
10.1155/2019/7847142
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发表时间：
2019-01-01
影响因子：
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通讯作者：
Bolboaca, Sorana D.
中科院分区：
文献类型：
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作者：
Boarescu, Paul-Mihai;Chirila, Ioana;Bolboaca, Sorana D.
姜黄素具有抗炎、抗氧化、抗癌和心血管保护作用。本研究旨在评价姜黄素纳米粒（CCNP）预处理对异丙肾上腺素（ISO）诱导的大鼠心肌梗死（MI）的影响。将五十六只Wistar-Bratislava白色大鼠随机分为八组，每组七只。姜黄素和姜黄素纳米颗粒以三种不同剂量（100 mg/kg体重（bw）、150 mg/kg bw和200 mg/kg bw）通过管饲给予15天。在第13天使用100 mg/kg bw ISO诱导MI，给药两次，第二次给药在首次给药后24小时。在ISO末次给药后24 h采集血样。在所有组中评价抗氧化、抗炎和心脏保护作用。所有剂量的CC和CCNP都通过防止心肌细胞肌酸激酶MB渗漏来提供心脏保护作用，其中CCNP的效果最好。与CC预处理相比，CCNP后所有氧化应激参数均显著改善。CCNP在限制MI后炎性细胞因子水平（如TNF-α、IL-6、IL-1 α、IL-1 β、MCP-1和RANTES）升高方面比CC更有效。CCNP预处理后MMP-2、MMP-9水平下降幅度大于CC。组织病理学检查，CCNP比CC更好地防止心肌坏死，减少间质水肿和中性粒细胞浸润。因此，通过纳米技术提高姜黄素的生物活性可能有助于限制心肌梗死后的心脏损伤。
Curcumin has anti-inflammatory, antioxidative, anticarcinogenic, and cardiovascular protective effects. Our study is aimed at evaluating the effects of pretreatment with curcumin nanoparticles (CCNP) compared to conventional curcumin (CC) on isoproterenol (ISO) induced myocardial infarction (MI) in rats. Fifty-six Wistar-Bratislava white rats were randomly divided into eight groups of seven rats each. Curcumin and curcumin nanoparticles were given by gavage in three different doses (100 mg/kg body weight (bw), 150 mg/kg bw, and 200 mg/kg bw) for 15 days. The MI was induced on day 13 using 100 mg/kg bw ISO administered twice, with the second dose 24 h after the initial dose. The blood samples were taken 24 h after the last dose of ISO. The antioxidant, anti-inflammatory, and cardioprotective effects were evaluated in all groups. All doses of CC and CCNP offered a cardioprotective effect by preventing creatine kinase-MB leakage from cardiomyocytes, with the best result for CCNP. All the oxidative stress parameters were significantly improved after CCNP compared to CC pretreatment. CCNP was more efficient than CC in limiting the increase in inflammatory cytokine levels (such as TNF-alpha, IL-6, IL-1 alpha, IL-1 beta, MCP-1, and RANTES) after MI. MMP-2 and MMP-9 levels decreased more after pretreatment with CCNP than with CC. CCNP better prevented myocardial necrosis and reduced interstitial edema and neutrophil infiltration than CC, on histopathological examination. Therefore, improving the bioactivity of curcumin by nanotechnology may help limit cardiac injury after myocardial infarction.