Effect of biphasic calcium phosphate scaffold porosities on odontogenic differentiation of human dental pulp cells
Effect of biphasic calcium phosphate scaffold porosities on odontogenic differentiation of human dental pulp cells
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DOI：
10.1177/0885328215625759
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发表时间：
2016-04-01
影响因子：
2.9
通讯作者：
Mahmood, Zuliani
中科院分区：
文献类型：
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作者：
AbdulQader, Sarah T.;Rahman, Ismail A.;Mahmood, Zuliani
不同孔隙率的磷酸钙（CaP）已被广泛并成功地用作成骨细胞的骨组织再生支架。然而，支架孔隙率对用于牙本质组织再生的人牙髓细胞的细胞活力和分化的影响并不为人所知。在本研究中，将平均孔径为300 μ m的羟基磷灰石/β-磷酸三钙比例为20/80的双相磷酸钙（BCP）支架制备成总孔隙率分别为25%、50%、65%和75%的BCP 1、BCP 2、BCP 3和BCP 4。这些支架的提取物进行了评估方面的细胞活力，增殖和分化的人牙髓细胞。与BCP 1和BCP 2相比，BCP 3和BCP 4表现出的高碱度以及更多的钙和磷酸根离子释放降低了人牙髓细胞的活力和增殖。BCP 2显著增加细胞活力和细胞增殖。然而，与用BCP 1、BCP 2和BCP 4提取物培养的细胞相比，用BCP 3提取物培养的细胞显示出高碱性磷酸酶（ALP）活性和成牙本质细胞相关基因、I型胶原α 1、牙本质基质蛋白-1和牙本质涎磷蛋白的高表达。结果突出了不同支架孔隙率对细胞微环境的影响，并证明65%孔隙率的BCP 3支架可以支持人牙髓细胞分化用于牙本质组织再生。
Calcium phosphates (CaP) of different porosities have been widely and successfully used as scaffolds with osteoblast cells for bone tissue regeneration. However, the effects of scaffold porosities on cell viability and differentiation of human dental pulp cells for dentin tissue regeneration are not well known. In this study, biphasic calcium phosphate (BCP) scaffolds of 20/80 hydroxyapatite to beta tricalcium phosphate ratio with a mean pore size of 300m were prepared into BCP1, BCP2, BCP3, and BCP4 of 25%, 50%, 65%, and 75% of total porosities, respectively. The extracts of these scaffolds were assessed with regard to cell viability, proliferation, and differentiation of human dental pulp cells. The high alkalinity, and more calcium and phosphate ions release that were exhibited by BCP3 and BCP4 decreased the viability and proliferation of human dental pulp cells as compared to BCP1 and BCP2. BCP2 significantly increased both cell viability and cell proliferation. However, the cells cultured with BCP3 extract revealed high alkaline phosphatase (ALP) activity and high expression of odontoblast related genes, collagen type I alpha 1, dentin matrix protein-1, and dentin sialophosphoprotein as compared to that cultured with BCP1, BCP2, and BCP4 extracts. The results highlight the effect of different scaffold porosities on the cell microenvironment and demonstrate that BCP3 scaffold of 65% porosity can support human dental pulp cells differentiation for dentin tissue regeneration.