OsMYC2 mediates numerous defence-related transcriptional changes via jasmonic acid signalling in rice.
OsMYC2 mediates numerous defence-related transcriptional changes via jasmonic acid signalling in rice.
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DOI：
10.1016/j.bbrc.2017.03.125
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发表时间：
2017-05
影响因子：
3.1
通讯作者：
Satoshi Ogawa;Ryouka Kawahara-Miki;K. Miyamoto;H. Yamane;H. Nojiri;Yoshimasa Tsujii;K. Okada
中科院分区：
文献类型：
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作者：
Satoshi Ogawa;Ryouka Kawahara-Miki;K. Miyamoto;H. Yamane;H. Nojiri;Yoshimasa Tsujii;K. Okada
茉莉酸（JA）在植物的许多事件中起着重要作用，特别是对病原体和昆虫的防御。碱性螺旋-环-螺旋（bHLH）转录因子MYC 2作为双子叶植物中JA信号传导的主要调节因子引起了人们的注意。然而，MYC 2如何在单子叶植物中发挥作用，包括农业上重要的作物，如栽培水稻，一直知之甚少。为了阐明水稻MYC 2（OsMYC 2）对茉莉酸诱导的转录修饰的综合效应，我们利用OsMYC 2基因敲低植株（osmyc 2 RNAi）进行了RNA测序。Inosmyc 2 RNAi，许多防御相关基因（例如几丁质酶和蛋白酶抑制剂）的JA诱导表达受到损害。特化代谢物（尤其是防御化合物）的生物合成途径的JA依赖性激活减少在theosmyc 2 RNAiline中也很明显。此外，在不同类型的转录因子，如MYB型因子的表达中注意到了实质性的变化，这可能描绘了OsMYC 2不仅在防御反应中而且在其他形态发生事件中的重要性。我们的研究结果提供了基本的信息，以了解整体功能的MYC 2在JA信号在单子叶植物，这可能会产生农业效益。
Jasmonic acid (JA) plays central roles in various events in plants, especially defence against pathogens and insects. The basic helix-loop-helix (bHLH) transcription factor MYC2 has attracted attention as a master regulator of JA signalling in dicotyledonous plants. However, how MYC2 functions in monocotyledonous plants, including agriculturally important crops such as cultivated rice, has been poorly understood. To elucidate the comprehensive effects of rice MYC2 (OsMYC2) on the JA-inducible transcriptional modifications, we performed RNA-sequencing by usingOsMYC2-knockdown plants (osmyc2RNAi). Inosmyc2RNAi, JA-inducible expression of many defence-related genes, for example chitinases and proteinase inhibitors, was compromised. Decrease in JA-dependent activation of the biosynthetic pathways of specialised metabolites, especially defence compounds, was also evident in theosmyc2RNAiline. Furthermore, a substantial change was noted in the expression of distinct types of transcription factors, such as MYB-type factors, likely depicting the importance of OsMYC2 in not only defence responses but also other morphogenetic events. Our findings provide fundamental information to understand the overall functions of MYC2 in JA signalling in monocotyledonous plants, which might yield agricultural benefits.