Creation of an ultra scale-down bioreactor mimic for rapid development of lignocellulosic enzymatic hydrolysis processes.
Creation of an ultra scale-down bioreactor mimic for rapid development of lignocellulosic enzymatic hydrolysis processes.
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DOI：
10.1002/jctb.4801
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发表时间：
2015-11
期刊：
影响因子：
--
通讯作者：
Lye GJ
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文献类型：
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作者：
Conroy N;Tebble I;Lye GJ
纤维素生物乙醇工艺涉及几个步骤，所有这些步骤都需要实验优化。对这项研究的一个重要帮助将是一个经过验证的超缩小（USD）模型，该模型可用于在工艺相关条件下使用有限的材料进行快速，广泛的筛选和优化实验。在这项工作中，使用30 mL的振荡锥形管作为USD模型的酶水解过程中建立。该方法被证明为水解的干酒糟与可溶物（DDGS）。在释放的糖的速率、组成和浓度方面，USD管的结果与4 L搅拌罐的结果非常相似，代表了80倍的规模减少。USD方法的效用说明调查的因素，可能会限制水解产率在高固体负荷。在水解过程中定期洗涤残留的固体，允许使用市售酶水解100%的可用糖。结果表明，USD系统成功地模仿了传统的搅拌槽在工业相关条件下的性能。该系统的效用通过其用于使用商业相关原料研究性能限制而得到证实。© 2015作者。Journal of Chemical Technology & Biotechnology由John Wiley & Sons Ltd代表化学工业协会出版。
Cellulosic bioethanol processes involve several steps, all of which require experimental optimisation. A significant aid to this research would be a validated ultra scale‐down (USD) model that could be used to perform rapid, wide ranging screening and optimisation experiments using limited materials under process relevant conditions. In this work, the use of 30 mL shaken conical tubes as a USD model for an enzymatic hydrolysis process is established. The approach is demonstrated for the hydrolysis of distillers' dried grains with solubles (DDGS). Results from the USD tubes closely mimic those obtained from 4 L stirred tanks, in terms of the rate, composition and concentrations of sugars released, representing an 80‐fold scale reduction. The utility of the USD approach is illustrated by investigating factors that may be limiting hydrolysis yields at high solids loadings. Washing the residual solids periodically during hydrolysis allowed 100% of the available sugar to be hydrolysed using commercially available enzymes. The results demonstrate that the USD system reported successfully mimics the performance of conventional stirred tanks under industrially relevant conditions. The utility of the system was confirmed through its use to investigate performance limitation using a commercially relevant feedstock. © 2015 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.