BIOCHEMICAL AND GENETIC-EVIDENCE FOR THE HEPATITIS-B VIRUS-REPLICATION STRATEGY
BIOCHEMICAL AND GENETIC-EVIDENCE FOR THE HEPATITIS-B VIRUS-REPLICATION STRATEGY
复制标题
DOI：
10.1126/science.3961490
复制
发表时间：
1986-04-25
期刊：
影响因子：
56.9
通讯作者：
VARMUS, HE
中科院分区：
文献类型：
--
作者：
SEEGER, C;GANEM, D;VARMUS, HE
乙型肝炎病毒通过 RNA 中间体的逆转录合成其开放环状 DNA 基因组。这个过程的细节已经通过使用哺乳动物乙型肝炎病毒来绘制 DNA 合成起始和终止位点的图谱，并探索在起始机制中涉及的短的、分离的直接重复序列（DR1 和 DR2）引入的突变的后果。第一条要合成的 DNA 链在 DR1 内起始，显然是由蛋白质引物启动的，并且完整的链具有短的末端冗余。相反，第二条 DNA 链以与 DR2 相邻的序列开始，但其 5'' 末端连接到包含 DR1 的寡核糖核苷酸；因此推定的RNA引物已被转置到DR2的位置。现在可以提出乙型肝炎病毒逆转录的详细策略，该策略可以与逆转录病毒使用的策略进行比较。
Hepatitis B viruses synthesize their open circular DNA genomes by reverse transcription of an RNA intermediate. The details of this process have been examined with the use of mammalian hepatitis B viruses to map the sites for initiation and termination of DNA synthesis and to explore the consequences of mutations introduced at short, separated direct repeats (DR1 and DR2) implicated in the mechanisms of initiation. The first DNA strand to be synthesized is initiated within DR1, apparently by a protein primer, and the completed strand has a short terminal redundancy. In contrast, the second DNA strand begins with the sequence adjacent to DR2, but its 5'' end is joined to an oligoribonucleotide that contains DR1; thus the putative RNA primer has been transposed to the position of DR2. It is now possible to propose a detailed strategy for reverse transcription by hepatitis B viruses that can be instructively compared with that used by retroviruses.