The protective role of ginsenoside compound K in porcine oocyte meiotic maturation failed caused by benzo(a)pyrene during in vitro maturation
The protective role of ginsenoside compound K in porcine oocyte meiotic maturation failed caused by benzo(a)pyrene during in vitro maturation
复制标题
人参皂苷化合物K对体外成熟过程中苯并芘引起的猪卵母细胞减数分裂成熟失败的保护作用
DOI：
10.1016/j.theriogenology.2020.07.006
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发表时间：
2020-11-01
期刊：
影响因子：
2.8
通讯作者：
Kang, Jin-Dan
中科院分区：
文献类型：
--
作者：
Luo, Zhao-Bo;Rahman, Saeed Ur;Kang, Jin-Dan
苯并（a）芘（BaP）是一种源自空气污染的污染物和致癌物，会对生殖系统，尤其是卵巢造成严重损害。人参常作为营养补充剂或草药用于食品和传统医学中。人参皂苷化合物K（CK）是人参的主要生物活性成分，具有非常独特的抗凋亡、抗氧化和抗炎活性，因此能保护细胞免受损伤。本研究旨在探究CK对BaP诱导的猪卵母细胞体外成熟抑制及其后续胚胎发育能力的影响。我们发现，在体外成熟过程中添加10毫克/毫升的CK，可显著提高经40微摩尔BaP处理造成损伤后的成熟率（P < 0.05）以及相关基因的表达水平。此外，添加CK后，活性氧（ROS）水平显著降低，ATP含量和线粒体膜电位（MMP）升高（P < 0.05）。在经BaP处理的卵母细胞中添加CK后，通过诱导多能性基因表达和抑制细胞凋亡，胚胎发育能力得到提升。添加10微克/毫升的CK，可通过增强抗氧化活性和改善BaP诱导损伤后的线粒体功能，提高猪卵母细胞的成熟度以及孤雌激活（33.01 对 20.92，P < 0.05）和体外受精（24.01 对 16.52，P < 0.05）后的胚胎发育能力。（C）2020爱思唯尔公司。保留所有权利。
Benzo(a)pyrene (BaP) is a pollutant and carcinogen derived from air pollution. It causes serious damage to reproductive system, especially ovary. Ginseng is always used in food and traditional medicine as a nutraceuticals or herbal medicine. Ginsenoside compound K (CK) is a major bioactive ingredient of ginseng, that shows very specific anti-apoptosis, anti-oxidant, and anti-inflammatory activities and thus, it protects cells from damage. The aim of this study was to investigate the effects of CK on the BaP-induced inhibition of the in vitro maturation of porcine oocytes and their subsequent embryonic development capacity. We found that supplementation with 10 mg mL(-1) CK during in vitro maturation significantly increased maturation rate (P < 0.05) and the expression level of related genes after damage induced by 40 mu M BaP treatment. In addition, reactive oxygen species (ROS) levels significantly decreased and ATP content and mitochondrial membrane potential (MMP) increased after CK supplementation (P < 0.05). The competence for embryonic development was improved by the induction of pluripotency gene expression and the inhibition of apoptosis after CK supplementation of BaP-treated oocytes. Supplementation with 10 mu g mL(-1) CK improved porcine oocyte maturation and subsequent embryonic development of parthenogenetic activation (33.01 vs. 20.92, P < 0.05) and in vitro fertilization (24.01 vs. 16.52, P < 0.05) by increasing antioxidant activity and improving mitochondrial function after BaP-induced damage. (C) 2020 Elsevier Inc. All rights reserved.