Effects of cycloheximide and tunicamycin on cell surface expression of pancreatic muscarinic acetylcholine receptors.
Effects of cycloheximide and tunicamycin on cell surface expression of pancreatic muscarinic acetylcholine receptors.
复制标题
放线菌酮和衣霉素对胰腺毒蕈碱乙酰胆碱受体细胞表面表达的影响。
DOI：
10.1016/0014-5793(90)81323-g
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发表时间：
1990
期刊：
影响因子：
3.5
通讯作者：
Habara,Y
中科院分区：
文献类型：
--
作者：
Hootman,SR;Verme,TB;Habara,Y
研究了糖基化在培养的豚鼠胰腺腺泡中毒蕈碱受体细胞表面表达中的重要性。恢复毒蕈碱受体人口后卡巴胆碱诱导下调被封锁放线菌酮，但不是衣霉素，虽然衣霉素减少[3H]甘露糖掺入腺泡大分子高达90%。衣霉素治疗也未能改变卡巴胆碱刺激淀粉酶分泌培养腺泡。这些结果表明，糖基化的腺亚型的毒蕈碱受体在胰腺腺泡细胞是不必要的插入质膜或其功能活性。
The importance of glycosylation in cell surface expression of muscarinic receptors in cultured guinea pig pancreatic acini was investigated. Recovery of the muscarinic receptor population after carbachol-induced down regulation was blocked by cycloheximide but not by tunicamycin, although tunicamycin reduced [3H]mannose incorporation into acinar macromolecules by up to 90%. Tunicamycin treatment also failed to alter carbachol stimulation of amylase secretion from cultured acini. These results indicate that glycosylation of the glandular subtype of muscarinic receptor in the pancreatic acinar cell is not necessary for its insertion in the plasma membrane or for its functional activity.