M(2)-SPECIFIC MUSCARINIC CHOLINERGIC RECEPTOR-MEDIATED INHIBITION OF CARDIAC REGULATORY PROTEIN-PHOSPHORYLATION
M(2)-SPECIFIC MUSCARINIC CHOLINERGIC RECEPTOR-MEDIATED INHIBITION OF CARDIAC REGULATORY PROTEIN-PHOSPHORYLATION
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DOI：
10.1152/ajpheart.1994.266.3.h1138
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发表时间：
1994-03-01
影响因子：
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通讯作者：
WATANABE, AM
中科院分区：
文献类型：
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作者：
GUPTA, RC;NEUMANN, J;WATANABE, AM
在豚鼠心室肌细胞中，10-100 nM异丙肾上腺素存在下，乙酰胆碱通过毒蕈碱胆碱受体作用降低受磷蛋白和肌钙蛋白I的磷酸化，而不降低腺苷3 '，5'-环磷酸（cAMP）水平或cAMP依赖性蛋白激酶活性比。腺苷脱氨酶（5 U/ml）存在时，乙酰胆碱的效果更明显，潜伏期短（10 s）。冈田酸，蛋白磷酸酶活性的抑制剂，阻断乙酰胆碱介导的抑制异丙肾上腺素刺激磷酸化的受磷蛋白。这表明，乙酰胆碱减少蛋白磷酸化的cAMP非依赖性机制在豚鼠心室肌细胞。
Acetylcholine acting via muscarinic cholinoceptors decreased phosphorylation of phospholamban and troponin I without reducing adenosine 3',5'-cyclic monophosphate (cAMP) levels or cAMP-dependent protein kinase activity ratio in the presence of 10-100 nM isoproterenol in guinea pig ventricular myocytes. The effect of acetylcholine was more pronounced when adenosine deaminase (5 U/ml) was present and incubation period was short (10 s). Okadaic acid, an inhibitor of protein phosphatase activity, blocked the acetylcholine-mediated inhibition of isoproterenol-stimulated phosphorylation of phospholamban. It is suggested that acetylcholine reduces protein phosphorylation by a cAMP-independent mechanism in guinea pig ventricular myocytes.