Plant regeneration from embryogenic cell suspensions and protoplasts of dessert banana cv. 'Da Jiao' (Musa paradisiacal ABB Linn.) via somatic embryogenesis
Plant regeneration from embryogenic cell suspensions and protoplasts of dessert banana cv. 'Da Jiao' (Musa paradisiacal ABB Linn.) via somatic embryogenesis
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从甜点香蕉品种的胚胎发生细胞悬浮液和原生质体再生植物。
DOI：
10.1007/s11627-010-9314-7
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发表时间：
2010-10-01
影响因子：
2.6
通讯作者：
Huang, Xue-Lin
中科院分区：
文献类型：
--
作者：
Dai, Xue-Mei;Xiao, Wang;Huang, Xue-Lin
香蕉（Musa paradisiacal ABB Linn.）是培育香蕉抗枯萎病新品种的理想种质。香蕉是我国南方广受欢迎的甜点香蕉品种，同时对古巴专化病菌4号生理小种具有很高的抗性。本研究建立了大椒胚性细胞悬浮系，并利用胚性细胞悬浮系的原生质体进行体细胞胚胎发生，获得了大椒胚性再生植株。幼穗在M1培养基上诱导出黄色易碎的愈伤组织，形成ECS。在不含2，4-二氯苯氧乙酸的M2培养基中进行预培养10 d，筛选ECS。在M3培养基中添加1.0 mg L ~（-1）脱落酸可使体细胞胚发生率提高2.6倍。从ECS中分离出原生质体，产量范围为5-6 × 10（6）/ml。用尖叶野蕉的ECS作饲养层培养1 mo后，约有0.35%的原生质体形成约100个细胞的微愈伤组织。Mas（AA）作为营养细胞。通过体细胞胚发生途径，从微愈伤组织中再生出健康植株（0.14%）。
The 'Da Jiao' cultivar of banana (Musa paradisiacal ABB Linn.) is an ideal germplasm to produce new banana varieties resistant to Fusarium oxysporum f. sp. cubense (FOC) race 4, for this cultivar is not only a popular dessert banana in south China, but also bears high resistance to FOC race 4. In this study, we established a homogeneous embryogenic cell suspension (ECS) of 'Da Jiao' and obtained regenerated plants from ECS-derived protoplasts via somatic embryogenesis. The ECS was initiated from yellow friable callus induced from immature male inflorescence on M1 medium. A pre-culture was used to select ECS in M2 medium without 2,4-dichlorophenoxyacetic acid for 10 d. Addition of 1.0 mg L-1 abscisic acid to M3 medium could enhance the frequency of somatic embryogenesis by about 2.6-fold. Protoplasts, with a yield range of 5-6 x 10(6) per milliliter, were isolated from the ECS. About 0.35% of the protoplasts formed microcallus, which contained about 100 cells, after 1 mo of feeder layer culture with ECS of Musa acuminate cv. Mas (AA) as nurse cells. Healthy plantlets (0.14%) were regenerated from the microcallus through somatic embryogenesis.