Mechanisms of Cong Rong Shu Jing Compound Effects on Endoplasmic Reticulum Stress in a Rat Model of Parkinson's Disease
Mechanisms of Cong Rong Shu Jing Compound Effects on Endoplasmic Reticulum Stress in a Rat Model of Parkinson's Disease
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丛容舒经复方对帕金森病模型大鼠内质网应激的影响机制
DOI：
10.1155/2020/1818307
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发表时间：
2020-05
影响因子：
--
通讯作者：
Cai Jing
中科院分区：
文献类型：
--
作者：
Lin Yao;Tang Lanfang;Huang Peizhen;Liu Ting;Zhong Jianan;Xu Qian;Li Xiyu;Lin Youning;Xiao Shaojian;Cai Jing
本研究探讨了丛容舒经 (CRSJ) 化合物对帕金森病 (PD) 大鼠模型内质网应激的影响。 40只大鼠颈后皮下注射鱼藤酮葵花油乳剂建立PD大鼠模型。将这些PD大鼠随机分为低、中、高剂量组（分别灌胃0.5、1和2g/kg CRSJ）和模型组（溶剂灌胃；每组10只）。此外，对照组和载体组各有10只大鼠（均接受CRSJ溶剂灌胃，载体组另外单独注射葵花籽油）。 PD模型建立后和CRSJ灌胃14天后进行两次牵引试验。分别使用免疫组织化学和高效液相色谱法评估黑质中酪氨酸羟化酶（TH-）阳性细胞的数量和多巴胺水平。 Western blotting检测α-突触核蛋白、内质网应激通路相关蛋白、脑多巴胺神经营养因子（CDNF）、中脑星形胶质细胞源性神经营养因子（MANF）和磷酸肌醇3激酶（PI3K）/蛋白激酶B（AKT）通路相关蛋白的表达水平。与模型组相比，CRSJ组黑质TH阳性细胞数量增多。 α-突触核蛋白和内质网应激途径相关蛋白葡萄糖调节蛋白 78、肌醇需求酶 1、凋亡信号调节激酶 1、磷酸化 c-Jun N 末端激酶和 caspase-12 的表达水平降低。然而，CRSJ 给药提高了神经营养因子 CDNF 和 MANF 以及 p-PI3K 和 p-AKT 的表达水平。 CRSJ化合物可以缓解PD大鼠的内质网应激，并在此动物模型中发挥保护作用。这些作用可能与神经营养因子表达增加和 PI3K/AKT 通路激活有关。
This study investigated the effects of the Cong Rong Shu Jing (CRSJ) compound on endoplasmic reticulum stress in a rat model of Parkinson's disease (PD). A total of 40 rats were subcutaneously injected with rotenone-sunflower oil emulsion into the back of the neck to establish a rat model of PD. These PD rats were randomly divided into low-, medium-, and high-dose groups (intragastric administration of 0.5, 1, and 2 g/kg CRSJ, respectively) and a model group (intragastric administration of the solvent; 10 rats per group). Furthermore, 10 rats each were attributed to the control and vehicle groups (both received intragastric administration of the CRSJ solvent, and the vehicle group were injected additionally with sunflower oil alone). A traction test was conducted two times, after the PD model establishment and after 14 days of CRSJ gavage. The numbers of tyrosine hydroxylase- (TH-) positive cells and the dopamine levels in the substantia nigra were assessed using immunohistochemistry and high-performance liquid chromatography, respectively. Western blotting detected the expression levels of alpha-synuclein, endoplasmic reticulum stress pathways-related proteins, cerebral dopamine neurotrophic factor (CDNF), mesencephalic astrocyte-derived neurotrophic factor (MANF), and phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) pathway-related proteins. Compared with the model group, the number of TH-positive cells in the substantia nigra was increased in the CRSJ groups. The expression levels of alpha-synuclein and the endoplasmic reticulum stress pathways-associated proteins glucose regulatory protein 78, inositol-requiring enzyme 1, apoptosis signal-regulating kinase 1, phosphorylated c-Jun N-terminal kinase, and caspase-12 were reduced. However, CRSJ administration elevated the expression levels of the neurotrophic factors CDNF and MANF, as well as those of p-PI3K and p-AKT. The CRSJ compound can relieve endoplasmic reticulum stress in PD rats and exerts protective effects in this animal model. These effects may be related to increased expression of neurotrophic factors and activation of the PI3K/AKT pathway.
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影响因子：
2.5
作者：
Khadrawy, Yasser A.;Salem, Ahmed M.;Hosny, Eman N.
通讯作者：
Hosny, Eman N.
影响因子：
5.3
作者：
Sherer, TB;Kim, JH;Greenamyre, JT
通讯作者：
Greenamyre, JT
影响因子：
3.3
作者：
Thayanidhi N;Helm JR;Nycz DC;Bentley M;Liang Y;Hay JC
通讯作者：
Hay JC
DOI：
10.1038/s41531-017-0015-3
发表时间：
2017
期刊：
NPJ Parkinson's disease
影响因子：
--
作者：
Klemann CJHM;Martens GJM;Sharma M;Martens MB;Isacson O;Gasser T;Visser JE;Poelmans G
通讯作者：
Poelmans G
影响因子：
56.9
作者：
Cooper, Antony A.;Gitler, Aaron D.;Lindquist, Susan
通讯作者：
Lindquist, Susan