Long term stability of paraoxonase-1 and high-density lipoprotein in human serum.
Long term stability of paraoxonase-1 and high-density lipoprotein in human serum.
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DOI：
10.1186/1476-511x-11-53
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发表时间：
2012-05-14
影响因子：
4.5
通讯作者：
Jansen EH
中科院分区：
文献类型：
--
作者：
Beekhof PK;Gorshunska M;Jansen EH
对氧磷酶-1（PON 1）是一种具有多种功能的酶，在临床和流行病学研究中受到越来越多的关注。有时样品在一定温度下储存较长时间。因此，必须检查PON 1活性的稳定性，并在储存后保持更长的时间。在本研究中，在3种常用温度（-20 ° C、-70 ° C和−196°C）下储存长达12个月期间，检测了人血清样品中PON 1活性的稳定性。结果发现，在−70°C和−196°C下储存12个月期间，PON 1活性的稳定性保持不变。在-20 °C下储存6个月后，其含量下降到原始值的约94%，但幅度较小，但具有统计学意义。尽管如此，T = 0和12个月时样本之间的排序保持不变。同样的温度依赖性被发现为相关的高密度脂蛋白。可以得出结论，-70 °C是保持PON 1活性至少一年的合适储存温度。不需要在液氮（−196°C）中低温储存。
Paraoxonase-1 (PON1) is an enzyme with numerous functions and receives an increasing interest in clinical and epidemiological studies. Sometimes samples are stored for longer periods at a certain temperature. Therefore the stability of PON1 activity must be checked and retained upon storage for longer periods. In this study the stability of PON1 activity has been tested in human serum samples during storage up to 12 months at 3 commonly used temperatures, -20°C, -70°C and −196°C. It was found that the stability of the PON1 activity is constant during 12 months of storage at −70°C and −196°C. Storage at −20°C resulted in a small but statistically significant decrease after 6 months to about 94% of its original value. Nonetheless, the rank order between the samples at T = 0 and 12 months remained the same. The same temperature dependence was found for the associated high-density lipoprotein. It can be concluded that −70°C is the right temperature for storage to maintain the PON1 activity for at least one year. Storage at a lower temperature in liquid nitrogen (−196°C) is not necessary.