Multiple dendritic cell populations activate CD4+ T cells after viral stimulation.
Multiple dendritic cell populations activate CD4+ T cells after viral stimulation.
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DOI：
10.1371/journal.pone.0001691
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发表时间：
2008-02-27
期刊：
影响因子：
3.7
通讯作者：
Belz GT
中科院分区：
文献类型：
--
作者：
Mount AM;Smith CM;Kupresanin F;Stoermer K;Heath WR;Belz GT
树突状细胞（DC）是连接先天性免疫系统和适应性免疫系统的异质性细胞群体。在病毒感染期间，CD8α DC在驱动CD8+ T细胞扩增方面发挥重要作用，有时是唯一的作用。这种对单个DC亚群的依赖是否也适用于CD4+ T细胞活化尚不清楚。我们使用直接离体抗原呈递试验来探测流式细胞术纯化的DC群体在通过不同途径感染流感病毒后驱动CD4+和CD8+ T细胞扩增的能力。本研究检测了皮肤和全身性流感病毒感染中非CD8 α DC群在CD8+和CD4+ T细胞扩增中的作用。我们证实，在体内，CD8+ T细胞的有效免疫应答主要由CD8α DC呈递抗原，但也可能涉及非CD8 α DC。相比之下，CD4+ T细胞反应更依赖于皮肤感染后从外周淋巴组织迁移的真皮DC和全身感染后脾脏中的CD4 DC的贡献。依赖于给药途径的DC亚群的CD4+ T细胞引发提高了疫苗接种方法可以针对引发辅助T细胞免疫的可能性。
Dendritic cells (DC) are a heterogeneous cell population that bridge the innate and adaptive immune systems. CD8α DC play a prominent, and sometimes exclusive, role in driving amplification of CD8+ T cells during a viral infection. Whether this reliance on a single subset of DC also applies for CD4+ T cell activation is unknown. We used a direct ex vivo antigen presentation assay to probe the capacity of flow cytometrically purified DC populations to drive amplification of CD4+ and CD8+ T cells following infection with influenza virus by different routes. This study examined the contributions of non-CD8α DC populations in the amplification of CD8+ and CD4+ T cells in cutaneous and systemic influenza viral infections. We confirmed that in vivo, effective immune responses for CD8+ T cells are dominated by presentation of antigen by CD8α DC but can involve non-CD8α DC. In contrast, CD4+ T cell responses relied more heavily on the contributions of dermal DC migrating from peripheral lymphoid tissues following cutaneous infection, and CD4 DC in the spleen after systemic infection. CD4+ T cell priming by DC subsets that is dependent upon the route of administration raises the possibility that vaccination approaches could be tailored to prime helper T cell immunity.
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DOI：
10.1084/jem.192.12.1685
发表时间：
2000-12-18
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
den Haan JM;Lehar SM;Bevan MJ
通讯作者：
Bevan MJ
影响因子：
5.4
作者：
BORROW, P;EVANS, CF;OLDSTONE, MBA
通讯作者：
OLDSTONE, MBA
影响因子：
4.4
作者：
Carter, Robert W.;Thompson, Clare;Tough, David F.
通讯作者：
Tough, David F.
CD8α(+)树突状细胞负责诱导外周组织相关抗原的自身耐受。
影响因子：
15.3
作者：
Belz, Gabrielle T;Behrens, Georg M N;Smith, Chris M;Miller, Jacques F A P;Jones, Claerwen;Lejon, Kristina;Fathman, C Garrison;Mueller, Scott N;Shortman, Ken;Carbone, Francis R;Heath, William R
通讯作者：
Heath, William R
影响因子：
4.4
作者：
Perry, JA;Rush, A;Avery, AC
通讯作者：
Avery, AC