3 SLOW MYOSIN HEAVY-CHAINS SEQUENTIALLY EXPRESSED IN DEVELOPING MAMMALIAN SKELETAL-MUSCLE
3 SLOW MYOSIN HEAVY-CHAINS SEQUENTIALLY EXPRESSED IN DEVELOPING MAMMALIAN SKELETAL-MUSCLE
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DOI：
10.1006/dbio.1993.1178
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发表时间：
1993-07-01
影响因子：
2.7
通讯作者：
BLAU, HM
中科院分区：
文献类型：
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作者：
HUGHES, SM;CHO, M;BLAU, HM
肌球蛋白重链（MyHC）亚型在哺乳动物发育过程中表现出惊人的多样性表达模式。使用一组单克隆抗体，识别肌球蛋白重链异构体上的不同表位，我们表明，存在于人类和大鼠骨骼肌中的慢收缩肌球蛋白重链的至少三种异构体。为了便于将我们的结果与使用不同抗体或物种获得的其他结果进行比较，我们鉴定了编码由三种慢抗体识别的表位的cDNA。使用这些试剂，我们表明，三个缓慢的MyHC亚型的表达的发病时间在妊娠早期是不同的。这一结果表明，一系列的MyHC转换起着重要的作用，在确定肌纤维功能在胎儿，新生儿和成人阶段。
Myosin heavy chain (MyHC) isoforms show a striking diversity of expression patterns during mammalian development. Using a set of monoclonal antibodies that recognize different epitopes on myosin heavy chain isoforms we show that there exist in human and rat skeletal muscle at least three isoforms of slow twitch myosin heavy chain. To facilitate a comparison of our results to others obtained using different antibodies or species, we have identified cDNAs encoding the epitopes recognized by the three slow antibodies. Using these reagents, we show that the onset of expression of three slow MyHC isoforms is temporally distinct during early gestation. This result suggests that a sequence of MyHC transitions plays an important role in determining muscle fiber function at fetal, neonatal, and adult stages.