Virus Assembly Pathways: Straying Away but Not Too Far
Virus Assembly Pathways: Straying Away but Not Too Far
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DOI：
10.1002/smll.202004475
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发表时间：
2020-11-25
期刊：
影响因子：
13.3
通讯作者：
Dragnea, Bogdan
中科院分区：
文献类型：
--
作者：
Bond, Kevin;Tsvetkova, Irina B.;Dragnea, Bogdan
无包膜RNA病毒遍布生命的所有领域。在细胞中，它们由病毒RNA和衣壳蛋白共同组装而成。病毒样颗粒可以在体外形成，其中几乎可以包装任何非同源聚阴离子货物。在存在无数其他聚阴离子物质的情况下，如何只有病毒RNA被选择用于体内包装一直是一个谜。通过电荷检测质谱和低温电子显微镜的组合，确定共组装雀麦花叶病毒（BMV）外壳蛋白和核酸寡聚体导致不同于二十面体病毒体的衣壳结构和化学计量。这些以前未知的壳结构是应变的，比天然的壳结构更不稳定。然而，它们含有大的天然结构片段，如果病毒基因组可用，则这些片段可以再循环以形成BMV病毒体。这种结构的存在表明细胞内包装过程可能存在以前未知的调节途径。
Non-enveloped RNA viruses pervade all domains of life. In a cell, they co-assemble from viral RNA and capsid proteins. Virus-like particles can form in vitro where virtually any non-cognate polyanionic cargo can be packaged. How only viral RNA gets selected for packaging in vivo, in presence of myriad other polyanionic species, has been a puzzle. Through a combination of charge detection mass spectrometry and cryo-electron microscopy, it is determined that co-assembling brome mosaic virus (BMV) coat proteins and nucleic acid oligomers results in capsid structures and stoichiometries that differ from the icosahedral virion. These previously unknown shell structures are strained and less stable than the native one. However, they contain large native structure fragments that can be recycled to form BMV virions, should a viral genome become available. The existence of such structures suggest the possibility of a previously unknown regulatory pathway for the packaging process inside cells.