Dual labeling with green fluorescent proteins for confocal microscopy
Dual labeling with green fluorescent proteins for confocal microscopy
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DOI：
10.1128/aem.66.1.413-418.2000
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发表时间：
2000-01-01
影响因子：
4.4
通讯作者：
Keasling, JD
中科院分区：
文献类型：
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作者：
Cowan, SE;Gilbert, E;Keasling, JD
我们报告了一种涉及两种绿色荧光蛋白(GFP)变体的双重标记技术，该技术与共聚焦显微镜兼容。两个激光被用来获得(I)混合细胞培养的图像，其中一种细胞含有GFPuv，另一种细胞含有GFP-mu2或GFP-mu3，以及(Ii)在同一细胞中同时含有GFPuv和GFP-mu2的单一细胞，这种方法有望用于监测基因表达，并作为一种非破坏性的原位共聚焦显微镜技术用于多物种生物膜的观察。
We report a dual labeling technique involving two green fluorescent protein (GFP) variants that is compatible with confocal microscopy. Two lasers were used to obtain images of (i) mixed cultures of cells, where one species contained GFPuv and another species contained GFPmut2 or GFPmut3, and (ii) a single species containing both GFPuv and GFPmut2 in the same cell, This method shows promise for monitoring gene expression and as a nondestructive and in situ technique for confocal microscopy of multispecies biofilms.