Extremely charged and incredibly soft: physical characterization of the pericellular matrix.
Extremely charged and incredibly soft: physical characterization of the pericellular matrix.
复制标题
充满电荷且极其柔软：细胞周基质的物理特征。
DOI：
10.1016/j.bpj.2013.01.035
复制
发表时间：
2013
影响因子：
3.4
通讯作者：
Janmey,PaulA
中科院分区：
文献类型：
--
作者：
vanOosten,AnneS;Janmey,PaulA
Most images and cartoons of cellmatrix or cell-cell adhesion show the plasma membrane of the cell connected to its binding partner by the nanomolar-scale extracellular domain of a transmembrane protein extending from a lipid bilayer to its equally small ligand in the adjacent cell or extracellular matrix. Cell adhesion, however, differs fundamentally from adhesion between liposomes or bubbles, even when mediated by the same adhesion proteins. Between the outermost edge of the cell membrane and that part of the extracellular matrix nearest to the cell lies a part of the cell that is largely overlooked: the pericellular matrix (PCM) or glycocalyx. The PCM was initially detected in electron microscopy studies of polysaccharide distribution as a fuzzy coat that covered, for example, the surface of cells lining the capillary lumen (Fig. 1), or the tips of microvilli in intestinal epithelia (1). This glycocalyx could extend several microns from the cell surface. Most light microscopy methods however, do not detect the PCM, and despite its robust appearance in electron micrographs, the PCM is generally too delicate to study by most rheology methods. The difficulties of studying the PCM in its native state have left the question of its potential mechanical role in cell function unresolved. In this issue of Biophysical Journal,
