Glomerular endothelial cells respond to calcium-mobilizing agonists with release of EDRF.
Glomerular endothelial cells respond to calcium-mobilizing agonists with release of EDRF.
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肾小球内皮细胞响应钙动员激动剂并释放 EDRF。
DOI：
10.1152/ajprenal.1990.258.5.f1295
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Ballermann,BJ
中科院分区：
文献类型：
--
作者：
Marsden,PA;Brock,TA;Ballermann,BJ
为了确定肾小球内皮细胞（glomerular endothelial cells， GEN）是否通过释放内皮源性松弛因子（endothelial -derived relax factor， EDRF）在局部控制肾小球功能中发挥作用，我们测定了几种激动剂对GEN胞质钙浓度（[Ca2+]i）和GEN EDRF释放的影响。缓激素、ATP、凝血酶和血小板活化因子（PAF）均以浓度依赖性的方式增加GEN中的[Ca2+]i，而血清素、乙酰胆碱、苯肾上腺素和内皮素-1则没有影响。将肾小球系膜细胞（GMC）与GEN增强的系膜细胞鸟苷3′，5′-环单磷酸（cGMP）含量共孵育5 - 6倍，缓激肽引起GMC cGMP含量在GEN存在而不是不存在的情况下进一步呈浓度依赖性增加。GEN依赖的缓激肽刺激的GMC cGMP积累被血红蛋白和亚甲基蓝所消除，被棉酚所钝化，并被超氧化物歧化酶所增强。其他能够增加GEN [Ca2+]i的激动剂也能在GEN存在而不存在的情况下刺激GMC cGMP的积累，因此培养的GEN释放一种刺激邻近系膜细胞cGMP积累的因子，具有EDRF的药理学特征。
To determine whether glomerular endothelial cells (GEN) may play a role in the local control of glomerular function by releasing endothelium-derived relaxing factor (EDRF), the effect of several agonists on GEN cytosolic calcium concentration ([Ca2+]i) and GEN EDRF release was determined. Bradykinin, ATP, thrombin, and platelet-activating factor (PAF) all increased [Ca2+]i in GEN in a concentration-dependent manner, whereas serotonin, acetylcholine, phenylephrine, and endothelin-1 were without effect. Coincubation of glomerular mesangial cells (GMC) with GEN augmented mesangial cell guanosine 3',5'-cyclic monophosphate (cGMP) content five- to sixfold, Bradykinin elicited a further concentration-dependent increase in GMC cGMP content in the presence but not absence of GEN. The GEN-dependent bradykinin-stimulated GMC cGMP accumulation was abolished by hemoglobin and methylene blue, blunted by gossypol, and augmented by superoxide dismutase. Other agonists capable of augmenting GEN [Ca2+]i also stimulated GMC cGMP accumulation in the presence but not in the absence of GEN. Thus cultured GEN release a factor that stimulates cGMP accumulation in adjacent mesangial cells which has the pharmacological characteristics of EDRF.