Staphylococcus aureus Skin Colonization Is Enhanced by the Interaction of Neutrophil Extracellular Traps with Keratinocytes
Staphylococcus aureus Skin Colonization Is Enhanced by the Interaction of Neutrophil Extracellular Traps with Keratinocytes
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DOI：
10.1016/j.jid.2019.10.017
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发表时间：
2020-05-01
影响因子：
6.5
通讯作者：
Schittek, Birgit
中科院分区：
文献类型：
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作者：
Bitschar, Katharina;Staudenmaier, Lena;Schittek, Birgit
金黄色葡萄球菌是一种兼性病原体，发现于皮肤和鼻腔表面。它通常在健康人的皮肤上不存在，但经常在特应性皮炎患者的皮肤上定居。在这里，我们研究了中性粒细胞在金黄色葡萄球菌皮肤定植的最初步骤中的功能作用，以及皮肤共生体如何调节金黄色葡萄球菌诱导的中性粒细胞向皮肤的募集。使用小鼠皮肤表面定植模型，我们发现剥离胶带引起的皮肤炎症导致中性粒细胞的快速募集，这与金黄色葡萄球菌皮肤定植增强有关。有趣的是，体内中性粒细胞的耗尽减少了金黄色葡萄球菌的定植，体外培养的原代人角质形成细胞与中性粒细胞共培养促进了金黄色葡萄球菌的黏附。我们证明，中性粒细胞胞外陷阱与角质形成细胞的相互作用是金黄色葡萄球菌皮肤定植增加的原因。最后，我们表明，表皮葡萄球菌作为皮肤微生物区系的一部分，可以减少金黄色葡萄球菌感染引起的中性粒细胞募集。这些数据表明，在炎症环境中，微生物区系介导的皮肤对金黄色葡萄球菌的保护受到抑制，在这种环境中，渗透的中性粒细胞释放的细胞外陷阱出人意料地促进了金黄色葡萄球菌皮肤定植的增强。
Staphylococcus aureus is a facultative pathogen found on skin and nasal surfaces. It is usually absent from the skin of healthy humans but frequently colonizes the skin of patients with atopic dermatitis. Here, we investigate the functional role of neutrophils in the initial steps of S. aureus skin colonization and how skin commensals modulate the S. aureuseinduced recruitment of neutrophils to the skin. Using an epicutaneous mouse skin colonization model, we show that skin inflammation induced by tape-stripping leads to a rapid recruitment of neutrophils, which correlates with enhanced S. aureus skin colonization. Interestingly, the depletion of neutrophils in vivo reduces S. aureus colonization, and in vitro coculture of primary human keratinocytes with neutrophils promotes S. aureus adherence. We demonstrate that the interaction of neutrophil extracellular traps with keratinocytes are responsible for the increased S. aureus skin colonization. Finally, we show that S. epidermidis as part of the skin microbiota can reduce the neutrophil recruitment induced by S. aureus infection. These data suggest that microbiota-mediated skin protection against S. aureus is dampened in an inflammatory environment in which neutrophil extracellular traps released by infiltrating neutrophils unexpectedly contribute to enhanced S. aureus skin colonization.