Identification of a novel WD repeat-containing gene predominantly expressed in developing and regenerating neurons
Identification of a novel WD repeat-containing gene predominantly expressed in developing and regenerating neurons
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DOI：
10.1093/oxfordjournals.jbchem.a022843
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发表时间：
2000-12-01
影响因子：
2.7
通讯作者：
Taga, T
中科院分区：
文献类型：
--
作者：
Kato, H;Chen, S;Taga, T
在目前的研究中，我们已经确定了一个新的基因，NDRP（神经元分化相关蛋白），它主要在发育和再生神经元中表达。预测的NDRP包含1019个氨基酸残基，在n端有6个WD重复序列，在c端有多个潜在的核定位信号（NLSs）。该分子与现有数据库中的其他分子没有明显的结构相似性。原位杂交和免疫组化结果显示，NRDP在胚胎发育期间的感觉神经元，如嗅觉上皮和视网膜神经层，以及围产期背根神经节中表达最高。该基因在完整的运动神经元如舌下神经中的表达未被检测到，但在切开后表达明显，提示该基因的产物可能参与了感觉神经元的发育和运动神经元的再生。
In the present study, we have identified a novel gene, NDRP (for neuronal differentiation-related protein), which is predominantly expressed in developing and regenerating neurons. The predicted NDRP comprises 1,019 amino acid residues and has 6 WD repeats in the N-terminal half and multiple potential nuclear localization signals (NLSs) at the C-terminal part. This molecule shows no significant structural similarity with any other molecules in available databases. In situ hybridization and immunohistochemistry revealed the highest expression of NRDP in sensory neurons, for instance, olfactory epithelia and neural layer of retina during embryonic development, as well as in perinatal dorsal root ganglions. The expression of this gene in intact motor neurons such as in the hypoglossal nerve was undetectable but became obvious after axotomy, These results suggest that the product of this gene might be involved in the development of sensory neurons as well as the regeneration of motor neurons.