Death of Quercus crispula by inoculation with adult Platypus quercivorus (Coleoptera: Platypodidae)
Death of Quercus crispula by inoculation with adult Platypus quercivorus (Coleoptera: Platypodidae)
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DOI：
10.1303/aez.2006.123
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发表时间：
2006-02-01
影响因子：
1.3
通讯作者：
Kobayashi, M
中科院分区：
文献类型：
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作者：
Kinuura, H;Kobayashi, M
将成年的鸭嘴兽（Platypus quercivorus）甲虫人工接种到日本栎（Quercus crispula）上。使用两种接种方法：通过移液管尖端均匀接种和通过释放到网中随机接种。均匀接种的五棵树中有四棵死了，随机接种的五棵树也都死了。九棵死树中有七棵表现出与目前橡树大规模死亡中相同的枯萎症状。从所有接种的濒死树中分离到一株已被确认为引起枯萎病的病原真菌-槲皮菌（Raffaelea quercivora），该真菌通常分离自槲皮菌（P. quercivorus）的菌丝体。死得更快的树显示出更高密度的甲虫画廊，成功地产生后代。我们发现，成功地产生后代的甲虫画廊的密度与边材变色率和R。quercivora。因此，我们清楚地证明了P quercivorus是R.日本栎树的大量死亡是由栎瘿螨的大规模攻击造成的。
Adult Platypus quercivorus beetles were artificially inoculated into Japanese oak trees (Quercus crispula). Two inoculation methods were used: uniform inoculation through pipette tips, and random inoculation by release into netting. Four of the five trees that were inoculated uniformly died, as did all five trees that were inoculated at random. Seven of the nine dead trees showed the same wilting symptoms seen in the current mass mortality of oak trees. Raffaelea quercivora, which has been confirmed to be the pathogenic fungus that causes wilt disease and is usually isolated from the mycangia of P quercivorus, was isolated from all of the inoculated dying trees. Trees that died faster showed a higher density of beetle galleries that succeeded in producing offspring. We found positive relationships between the density of beetle galleries that succeeded in producing offspring and the rate of discoloration in the sapwood and the isolation rate of R. quercivora. Therefore, we clearly demonstrated that P quercivorus is a vector of R. quercivora, and that the mass mortality of Japanese oak trees is caused by mass attacks of P quercivorus.