Intrinsic fluorescence from DNA can be enhanced by metallic particles
Intrinsic fluorescence from DNA can be enhanced by metallic particles
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DOI：
10.1006/bbrc.2001.5445
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发表时间：
2001-09-07
影响因子：
3.1
通讯作者：
Gryczynski, I
中科院分区：
文献类型：
--
作者：
Lakowicz, JR;Shen, B;Gryczynski, I
DNA 的高灵敏度检测对于基因组学至关重要。 DNA 的内在荧光非常弱，几乎所有检测 DNA 的方法都依赖于使用外源荧光探针。我们证明，DNA 的固有发射可以通过空间接近银岛薄膜而增强许多倍。银岛是惰性基底上亚波长大小的金属银斑块。时间分辨测量显示银岛附近固有 DNA 发射的寿命缩短。这些强度增加和寿命缩短的结果表明金属引起的 DNA 碱基辐射速率衰减增加。 DNA 碱基和其他荧光团的辐射衰减率增加的可能性表明，基于金属诱导的弱荧光物质的荧光量子产率增加的多种 DNA 测量和其他生物医学测定。 (C) 2001 年学术出版社。
High sensitivity detection of DNA is essential for genomics. The intrinsic fluorescence from DNA is very weak and almost all methods for detecting DNA rely on the use of extrinsic fluorescent probes. We show that the intrinsic emission from DNA can be enhanced many-fold by spatial proximity to silver island films. Silver islands are subwavelength size patches of metallic silver on an inert substrate. Time-resolved measurements show a decreased lifetime for the intrinsic DNA emission near the silver islands. These results of increased intensity and decreased lifetime indicate a metal-induced increase in the radiative rate decay of the DNA bases. The possibility of increased radiative decay rates for DNA bases and other fluorophores suggest a wide variety of DNA measurements and other biomedical assays based on metal-induced increases in the fluorescence quantum yield of weakly fluorescent substances. (C) 2001 Academic Press.