Gestational profile of Na+/H+ exchanger and Cl-/HCO-3 anion exchanger MRNA expression in placenta using real-time QPCR
Gestational profile of Na+/H+ exchanger and Cl-/HCO-3 anion exchanger MRNA expression in placenta using real-time QPCR
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DOI：
10.1016/j.placenta.2004.05.005
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发表时间：
2005-01-01
期刊：
影响因子：
3.8
通讯作者：
Sibley, CP
中科院分区：
文献类型：
--
作者：
Lacey, HA;Nolan, T;Sibley, CP
母体血流的开始（妊娠10-12周）导致胎盘氧合增加。我们研究了Na+/H+交换器（NHE）和Cl-/HCO3-阴离子交换器（AE）的表达是否随着血流量的增加而改变，它们被认为在维持合胞滋养细胞的细胞内pH和胎儿pH稳态中起重要作用。采用实时荧光定量PCR检测妊娠早期（6-9周）和晚期（10-13周）和足月（38-40周）胎盘中NHE （NHE 1、2、3）和AE (AE 1、2)mRNA的稳态表达水平。同时检测β - actin、IF2B和GAPDH mRNA。这些基因在孕早期组和孕晚期组之间的表达没有显著差异。然而，NHE2 （p < 0.001）和GAPDH (p < 0.05) mRNA的表达在妊娠早期和足月期间显著增加了18倍和3.7倍。总之，本研究提供了额外的证据，证明GAPDH是一个不适合用于胎盘转录水平正常化的管家基因。绒毛胎盘中NHE和AE的表达不随母体血流的开始而改变。然而，NHE2转录本似乎在妊娠期受到调控，这可能有助于NHE活性的变化。(C) 2004 Elsevier Ltd.版权所有。
The onset of maternal blood flow (10-12 weeks gestation) results in increased oxygenation of the placenta. We investigated whether the expressions of Na+/H+ exchanger (NHE) and Cl-/HCO3- anion exchanger (AE), thought to have an important role in maintaining intracellular pH of the syncytiotrophoblast and fetal pH homeostasis, are altered at the same time as this increase in blood flow. Real-time quantitative PCR was used to examine steady state levels of NHE (NHE 1, 2, 3) and AE (AE 1, 2) mRNA expression in early (6-9 weeks) and late (10-13 weeks) first trimester and full-term (38-40 weeks) placentas. beta-Actin, IF2B and GAPDH mRNA was also measured. None of the genes showed a significant difference in expression between the early and late first trimester groups. However, NHE2 (p < 0.001) and GAPDH (p < 0.05) mRNA expression significantly increased 18- and 3.7-fold between early first trimester and term. In conclusion, this study provides additional evidence that GAPDH is an unsuitable housekeeping gene for normalization of transcript levels in placenta. The expression of NHE and AE in the villous placenta is not altered concomitant with the onset of maternal blood flow. However, NHE2 transcripts appear to be gestationally regulated, which may contribute to changes in NHE activity. (C) 2004 Elsevier Ltd. All rights reserved.