An Integrated Bioinformatics Analysis Reveals Divergent Evolutionary Pattern of Oil Biosynthesis in High- and Low-Oil Plants.
An Integrated Bioinformatics Analysis Reveals Divergent Evolutionary Pattern of Oil Biosynthesis in High- and Low-Oil Plants.
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综合生物信息学分析揭示高油和低油植物油生物合成的不同进化模式
DOI：
10.1371/journal.pone.0154882
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Zhang YM
中科院分区：
文献类型：
--
作者：
Zhang L;Wang SB;Li QG;Song J;Hao YQ;Zhou L;Zheng HQ;Dunwell JM;Zhang YM
种子油提供了对人类很重要的可再生食物、生物燃料和工业原料来源。虽然已经鉴定了许多酰基脂质代谢的基因和途径，但对于高油植物中是否存在高油含量的特定机制知之甚少。基于4种高油双子叶植物（20~50%）和3种低油禾本科植物（<3%）种子含油量的显著差异，本研究采用比较基因组、转录组和差异表达分析方法探讨了这一差异的机制。从7个物种的252，443个基因中鉴定出4，051个双子叶大豆特异基因，其中54个基因直接参与酰基脂质代谢，93个基因与酰基脂质代谢相关。在93个双子叶植物特异表达的基因中，包括CBM 20-like SBD和GPT 2在内的42个和27个基因分别参与碳水化合物的降解和转运。在种子油快速积累期高表达的40个基因主要参与脂肪酸的初始合成、三酰甘油酯的组装和油体的形成，如ACC酶、PP、DGAT 1、PDAT 1、OLEs和STERO，这些基因在高油和低油大豆品种间也存在差异表达。系统发育分析表明，油质蛋白基因在高油双子叶植物和低油禾本科植物中的复制和丢失模式存在明显差异。此外，种子特异的GmGRF 5，ABI 5和GmTZF 4被预测为种子油积累的候选调节剂。本研究为进一步研究油脂生物合成和种子含油量的遗传改良奠定了基础。
Seed oils provide a renewable source of food, biofuel and industrial raw materials that is important for humans. Although many genes and pathways for acyl-lipid metabolism have been identified, little is known about whether there is a specific mechanism for high-oil content in high-oil plants. Based on the distinct differences in seed oil content between four high-oil dicots (20~50%) and three low-oil grasses (<3%), comparative genome, transcriptome and differential expression analyses were used to investigate this mechanism. Among 4,051 dicot-specific soybean genes identified from 252,443 genes in the seven species, 54 genes were shown to directly participate in acyl-lipid metabolism, and 93 genes were found to be associated with acyl-lipid metabolism. Among the 93 dicot-specific genes, 42 and 27 genes, including CBM20-like SBDs and GPT2, participate in carbohydrate degradation and transport, respectively. 40 genes highly up-regulated during seed oil rapid accumulation period are mainly involved in initial fatty acid synthesis, triacylglyceride assembly and oil-body formation, for example, ACCase, PP, DGAT1, PDAT1, OLEs and STEROs, which were also found to be differentially expressed between high- and low-oil soybean accessions. Phylogenetic analysis revealed distinct differences of oleosin in patterns of gene duplication and loss between high-oil dicots and low-oil grasses. In addition, seed-specific GmGRF5, ABI5 and GmTZF4 were predicted to be candidate regulators in seed oil accumulation. This study facilitates future research on lipid biosynthesis and potential genetic improvement of seed oil content.