Quantification of sporozoite invasion, migration, and development by microscopy and flow cytometry.

Quantification of sporozoite invasion, migration, and development by microscopy and flow cytometry.
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通过显微镜和流式细胞术定量子孢子的侵袭、迁移和发育。

DOI:
10.1007/978-1-62703-026-7_27
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发表时间:
2013
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
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通讯作者:
Mota,MariaM
Mota,MariaM
中科院分区:
--
文献类型:
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作者:
Sinnis,Photini;DeLaVega,Patricia;Coppi,Alida;Krzych,Urszula;Mota,MariaM

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体外检测对于更好地了解疟疾感染的初始步骤具有重要作用。在这一部分中,我们描述了基于显微镜和基于流式细胞术的子孢子对啮齿动物疟疾寄生虫伯氏疟原虫和约氏疟原虫以及人类疟疾寄生虫恶性疟原虫的入侵、迁移和发育分析。
There is an important role for in vitro assays to better understand the initial steps of malaria infection. In this section, we describe both microscopy-based and flow cytometry-based sporozoite invasion, migration and development assays with the rodent malaria parasites,Plasmodium bergheiandPlasmodium yoelii, and the human malaria parasite,Plasmodium falciparum.