The Distribution of a Phage-Related Insertion Sequence Element in the Cyanobacterium, Microcystis aeruginosa
The Distribution of a Phage-Related Insertion Sequence Element in the Cyanobacterium, Microcystis aeruginosa
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DOI：
10.1264/jsme2.me10125
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发表时间：
2010-12-03
影响因子：
2.2
通讯作者：
Sako, Yoshihiko
中科院分区：
文献类型：
--
作者：
Kuno, Sotaro;Yoshida, Takashi;Sako, Yoshihiko
蓝藻噬菌体Ma-LMM 01，特异性感染铜绿微囊藻，具有插入序列（IS）元件，我们命名为IS 607-cp显示出高度的核苷酸相似性，在基因组中的对应的蓝藻蓝藻。用PCR方法检测铜绿假单胞菌IS 607-cp的存在，并在菌株NIES 90、NIES 112、NIES 604和RM 6中检测到该元件。热不对称交错PCR（TAIL-PCR）结果显示，这些菌株均含有多个IS 607-cp拷贝。根据插入位点的不同，将部分ISs分为3种类型：IS 607-cp-1常见于NIES 90、NIES 112和NIES 604，IS 607-cp-2和IS 607-cp-3分别特异于NIES 90和RM 6。这种多样性可能反映了IS 607-cp的复制转座。Ma-LMM 01中的IS 607-cp序列显示出与M中发现的那些序列的强亲和力。铜绿假单胞菌和蓝杆藻属（Cyanothece spp.）从各种细菌的对应物推断出的系统发育树中。这表明IS 607-cp在蓝细菌和其噬藻体之间转移。我们讨论了Ma-LMM 01相关的转录因子作为IS元件的供体的潜在作用，这些IS元件可能介导IS 607-cp的转移，从而部分有助于M.铜绿。
The cyanophage Ma-LMM01, specifically-infecting Microcystis aeruginosa, has an insertion sequence ( IS) element that we named IS607-cp showing high nucleotide similarity to a counterpart in the genome of the cyanobacterium Cyanothece sp. We tested 21 strains of M. aeruginosa for the presence of IS607-cp using PCR and detected the element in strains NIES90, NIES112, NIES604, and RM6. Thermal asymmetric interlaced PCR (TAIL-PCR) revealed each of these strains has multiple copies of IS607-cp. Some of the ISs were classified into three types based on their inserted positions; IS607-cp-1 is common in strains NIES90, NIES112 and NIES604, whereas IS607-cp-2 and IS607-cp-3 are specific to strains NIES90 and RM6, respectively. This multiplicity may reflect the replicative transposition of IS607-cp. The sequence of IS607-cp in Ma-LMM01 showed robust affinity to those found in M. aeruginosa and Cyanothece spp. in a phylogenetic tree inferred from counterparts of various bacteria. This suggests the transfer of IS607-cp between the cyanobacterium and its cyanophage. We discuss the potential role of Ma-LMM01-related phages as donors of IS elements that may mediate the transfer of IS607-cp; and thereby partially contribute to the genome plasticity of M. aeruginosa.