Introduction to a new cell transplantation platform via recombinant peptide petaloid pieces and its application to islet transplantation with mesenchymal stem cells
Introduction to a new cell transplantation platform via recombinant peptide petaloid pieces and its application to islet transplantation with mesenchymal stem cells
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DOI：
10.1111/tri.12810
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发表时间：
2016-09-01
影响因子：
3.1
通讯作者：
Yoshioka, Yasuhiro
中科院分区：
文献类型：
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作者：
Nakamura, Kentaro;Iwazawa, Reiko;Yoshioka, Yasuhiro
移植细胞中的细胞死亡簇仍然是再生策略的关键障碍。本研究描述了一种新型的细胞移植平台(CellSaic)，该平台由人骨髓间充质干细胞(HMSCs)和花瓣状重组多肽(RCP)组成，通过将细胞排列成马赛克来防止细胞死亡。当hMSC CellSaics被植入NOD/SCID小鼠的皮下时，hMSC CellSaics防止了细胞死亡并加速了移植物中的血管生成，与仅植入细胞球体的研究结果相比。此外，我们还研究了CellSAIC在200只大鼠胰岛与2×10(5)hMSCs皮下联合移植到糖尿病小鼠中的应用。血糖1M时，单纯胰岛移植组为398+/-30 mg/dl，胰岛+hMSCs组为180+/-65 mg/dl。另一方面，hMSCs-CellSaic组胰岛细胞浓度为129+/-15 mg/dl，糖耐量显著改善(P<；0.05)。此外，我们还发现RCP瓣状碎片的表面纹理在移植物存活和血管生成中起着重要作用。预计CellSaic将被用作细胞移植和组织再生的新平台。
Cell death cluster in transplanted cells remains a critical obstacle for regeneration strategies. This study describes a novel platform for cell transplantation (CellSaic) consisting of human mesenchymal stem cells (hMSCs) and petaloid pieces of recombinant peptide (RCP), which can prevent cell death by arranging the cells in a mosaic. When hMSC CellSaics were subcutaneously implanted into NOD/SCID mice, hMSC CellSaics prevented cell death and accelerated angiogenesis in the graft, compared to the findings obtained on solely implanting cell spheroids. Additionally, we examined the application of CellSaic for subcutaneous cotransplantation of 200 rat islets with 2 x 10(5) hMSCs into diabetic mice. As the results of blood glucose levels at 1 M, the islet-only group was 398 +/- 30 mg/dl and the islets with hMSCs group were 180 +/- 65 mg/dl. On the other hand, the islets with hMSCs CellSaic group showed 129 +/- 15 mg/dl and significantly improved glucose tolerance (P < 0.05). Additionally, we showed that the surface texture of the RCP petaloid pieces played an important role in graft survival and angiogenesis. It is anticipated that CellSaic will be used as a new platform for cell transplantation and tissue regeneration.