CEPH family 1362 STR database: an online resource for characterization of PCR products using electrospray ionization mass spectrometry.
CEPH family 1362 STR database: an online resource for characterization of PCR products using electrospray ionization mass spectrometry.
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CEPH 系列 1362 STR 数据库：使用电喷雾电离质谱表征 PCR 产物的在线资源。
DOI：
10.1016/s1044-0305(01)00326-9
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发表时间：
2002
影响因子：
3.2
通讯作者：
Muddima,DavidC
中科院分区：
文献类型：
--
作者：
Null,AllisonP;Muddima,DavidC
我们建立了一个在线数据库，以验证用于基因分型的电喷雾电离质谱 (ESI-MS) 并公开我们实验室开发的用于通过 ESI-MS 表征 PCR 产物的程序。使用 ESI 傅里叶变换离子回旋共振质谱 (FTICR-MS) 获得的短串联重复 (STR) 位点衍生的基因型已发布于 CEPH 家族 1362 谱系的 15 个成员。该网站提供具体信息，例如 PCR 参数、PCR 产物净化方法和 ESI 溶液成分，以便其他实验室能够重现我们的数据。提供了相关网站的链接，以整合有关 CEPH 家族、STR 基因分型和质谱的信息。该数据库目前可在 http://www.人们。维库。 edu/~ dcmuddim/genotype/ 将定期更新来自其他 STR 位点的基因型，包括 PCR 参数以及随着进一步开发的完成而进行的 PCR 清理策略。
An online database has been established in order to validate electrospray ionization mass spectrometry (ESI-MS) for genotyping and to publicize the procedures developed in our laboratory for the characterization of PCR products by ESI-MS. Genotypes derived from short tandem repeat (STR) loci that were obtained using ESI Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) have been posted for fifteen members of the CEPH family 1362 pedigree. The website provides specific information such as PCR parameters, PCR product cleanup approaches, and ESI solution compositions to enable other laboratories to reproduce our data. Links are provided to related websites in an effort to integrate information regarding the CEPH family, STR genotyping, and mass spectrometry. The database, currently available at http://www. people. vcu. edu/~ dcmuddim/genotype/will be routinely updated with genotypes from additional STR loci including PCR parameters as well as PCR cleanup strategies as further developments are completed.