Preparation of berberine hydrochloride long-circulating liposomes by ionophore A23187-mediated ZnSO4 gradient method
Preparation of berberine hydrochloride long-circulating liposomes by ionophore A23187-mediated ZnSO4 gradient method
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DOI：
10.1016/j.ajps.2013.09.009
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发表时间：
2013-08-01
影响因子：
10.2
通讯作者：
Deng, Yihui
中科院分区：
文献类型：
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作者：
Luo, Xiang;Li, Jing;Deng, Yihui
本研究旨在制备盐酸小檗碱长循环脂质体，优化制剂配方和工艺参数，并考察不同因素对包封率的影响。利用离子载体A23187建立跨膜离子梯度来制备盐酸小檗碱脂质体。通过阳离子交换树脂分离游离药物和脂质体药物，然后采用紫外分光光度法测定脂质体内盐酸小檗碱的含量。当药脂比为1∶20时，盐酸小檗碱脂质体的优化包封率为94.3%±2.1%，平均粒径为146.9 nm±3.2 nm。结果表明，离子载体A23187介导的硫酸锌梯度法适用于制备盐酸小檗碱脂质体，可获得理想的包封率和载药量。版权所有(C) 2013沈阳药科大学。由爱思唯尔出版集团制作和托管。保留所有权利。
The aim of the study was to prepare berberine hydrochloride long-circulating liposomes and optimize the formulation and process parameters, and investigate the influence of different factors on the encapsulation efficiency. Berberine hydrochloride liposomes were prepared in response to a transmembrane ion gradient that was established by ionophore A23187. Free and liposomal drug were separated by cation exchange resin, and then the amount of intra-liposomal berberine hydrochloride was determined by UV spectrophotometry. The optimized encapsulation efficiency of berberine hydrochloride liposomes was 94.3% +/- 2.1% when the drug-to-lipid ratio was 1: 20, and the mean diameter was 146.9 nm +/- 3.2 nm. As a result, the ionophore A23187-mediated ZnSO4 gradient method was suitable for the preparation of berberine hydrochloride liposomes that we could get the desired encapsulation efficiency and drug loading. (C) 2013 Shenyang Pharmaceutical University. Production and hosting by Elsevier B.V. All rights reserved.