Endothelium-dependent vasodilatation effects of the essential oil from Fructus Alpiniae Zerumbet (EOFAZ) on rat thoracic aortic rings in vitro
Endothelium-dependent vasodilatation effects of the essential oil from Fructus Alpiniae Zerumbet (EOFAZ) on rat thoracic aortic rings in vitro
复制标题
高山姜精油 (EOFAZ) 对大鼠胸主动脉环的体外内皮依赖性血管舒张作用
DOI：
10.1016/j.phymed.2012.12.014
复制
发表时间：
2013-03-15
期刊：
影响因子：
7.9
通讯作者：
Shen, Xiang Chun
中科院分区：
文献类型：
--
作者：
Tao, Ling;Hu, Han Shuai;Shen, Xiang Chun
高良姜（Fructus Alpiniae Zerumbet，FAZ）是贵州地区广泛应用于治疗血管疾病的中草药，其挥发油是其具有舒张血管作用的活性成分之一，尤其是其挥发油成分与叶有显著差异。研究了FAZ挥发油（EOFAZ）的扩血管作用及其机制。用去甲肾上腺素（NE，1.0 μ M）或KCl（60 mM）预孵育的大鼠胸主动脉环，EOFAZ对内皮有明显的舒张作用，呈浓度依赖性（1.14-72.96 μ g/ml）。非选择性一氧化氮合酶抑制剂L-NAME，以及可溶性鸟苷酸环化酶抑制剂MB，减弱内皮完整大鼠胸主动脉环EOFAZ的舒张。然而，用吲哚美辛（Indo）抑制环氧合酶或普萘洛尔（Prop）抑制β-去甲肾上腺素能，对血管舒张作用无显著影响。本研究结果首次证实EOFAZ的扩血管作用依赖于内皮细胞和浓度，其机制涉及NOS-cGMP系统。相反，前列环素和β-肾上腺素受体可能与EOFAZ诱导的血管舒张无关。Crown版权所有（C）2012由Elsevier GmbH发布。All rights reserved.
Fructus Alpiniae Zerumbet (FAZ) is an herb widely used to treat vascular disorders in Guizhou province, China, the essential oil has been identified as one of it vasodilation effect active components, and especial, the composition was significantly difference from the leaves. Vasodilation effects and mechanism of essential oil from FAZ (EOFAZ) were investigated. The EOFAZ showed significant vasodilation effect on endothelium-with rat thoracic aortic rings preincubated with norepinephrine (NE, 1.0 mu M) or KCl (60 mM) in a concentration-dependent manner (1.14-72.96 mu g/ml). The non-selective nitric oxide synthase inhibitor L-NAME, as well as the soluble guanylate cyclase inhibitor MB, attenuated the relaxation of EOFAZ in endothelium-intact rat thoracic aortic rings. However, there were not significantly affected the vasodilation effects pretreated with cyclooxygenase inhibition by indomethacin (Indo) or beta-noradrenergic inhibition by propranolol (Prop). The present results first demonstrated that vasodilation effect of EOFAZ depending upon the endothelium and concentration, and the mechanism involvement of NOS-cGMP system. In contrast, prostacyclin and beta-adrenoceptor may not be associated with EOFAZ-induced vasorelaxation. Crown Copyright (C) 2012 Published by Elsevier GmbH. All rights reserved.