Efficient autofocus method for sequential automatic capturing of high-magnification microscopic images
Efficient autofocus method for sequential automatic capturing of high-magnification microscopic images
复制标题
用于连续自动捕获高倍显微图像的高效自动对焦方法
DOI：
10.3788/col201311.121102
复制
发表时间：
2013
影响因子：
3.5
通讯作者：
Edgar Valdez
中科院分区：
文献类型：
--
作者：
Santiago Tello;Francisco Flores;Jesús Bescós;Edgar Valdez
这封信介绍了一种自动对焦（AF）方法，用于定位高放大倍率显微镜透镜，该透镜可从单个移动载玻片自动捕获数百张图像。这些图像由农村地区的一个移动的诊所拍摄，随后由远程专家自动处理和修改。此过程需要高聚焦精度以使图像处理技术能够实现适当的结果。低聚焦时间也是系统工作所必需的。我们提出了一种新的方法，结合了两个重点措施与适应搜索计划，以科普这两个约束。
This letter presents an autofocus (AF) method to position a high-magnification microscope lens that automatically captures hundreds of images from a single moving slide. These images are taken by a mobile clinic unit in a rural location, and are later automatically processed and revised by a remote specialist. This process requires high focus precision to enable image processing techniques to achieve proper results. Low focusing times are also required for the system to be operative. We propose a novel method that combines two focus measures with an adapted searching scheme to cope with both constraints.