blaCTX-M-15-carrying Escherichia coli and Salmonella isolates from livestock and food in Germany
blaCTX-M-15-carrying Escherichia coli and Salmonella isolates from livestock and food in Germany
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DOI：
10.1093/jac/dku270
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发表时间：
2014-11-01
影响因子：
5.2
通讯作者：
Guerra, Beatriz
中科院分区：
文献类型：
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作者：
Fischer, Jennie;Rodriguez, Irene;Guerra, Beatriz
产CTX-M-15 β-内酰胺酶的大肠杆菌和沙门氏菌分离株主要来源于德国牲畜和食品。通过PCR扩增/测序检查了来自联邦风险评估研究所的常规和监测提交资料（2003-12）以及不同国家靶向研究（2011-12）的对第三代头孢菌素耐药的大肠杆菌（526，主要为沙门氏菌）和沙门氏菌（151）非人分离株中bla（CTX-M-15）基因的存在。通过基因芯片和PCR扩增筛选出其他抗性和毒力基因。E.对携带bla（CTX-M-15）的大肠杆菌分离株进行系统发育分类、PFGE和多位点序列分型（MLST）。通过复制子分型、质粒MLST、S1核酸酶PFGE和Southern印迹杂交等方法对bla（CTX-M-15）质粒进行了分析。产CTX-M-15菌株中，大肠杆菌（家畜、食品和玩具，4.0%）和沙门氏菌（马和猪，1.3%）为阳性。E.大肠杆菌分离株主要归属于序列类型ST 678的三个克隆谱系（德国爆发的产肠聚集性志贺毒素的大肠杆菌;大肠杆菌O 104：H4;鲑鱼、黄瓜和玩具）、ST 410（家禽、猪和牛养殖场）和ST 167/617（猪养殖场和火鸡肉）。bla（CTX-M-15）基因位于IncI 1和多复制子IncF质粒上或大肠杆菌染色体上。在德国，来自非人类来源的产CTX-M-15分离株的流行率仍然很低。而bla（CTX-M-15）基因存在于多药耐药大肠杆菌中。在家畜和食品中具有致病潜力的大肠杆菌克隆。由于染色体携带，bla（CTX-M-15）基因的维持值得注意。牲畜和人类之间（双向）交换产生CTX-M-15的分离株或质粒的可能性值得持续监测。
The characterization of CTX-M-15 beta-lactamase-producing Escherichia coli and Salmonella isolates originating mainly from German livestock and food.E. coli (526, mainly commensals) and Salmonella (151) non-human isolates resistant to third-generation cephalosporins, originating from routine and monitoring submissions (2003-12) to the Federal Institute for Risk Assessment and different national targeted studies (2011-12), were examined for the presence of bla(CTX-M-15) genes by PCR amplification/sequencing. Additional resistance and virulence genes were screened by DNA microarray and PCR amplification. E. coli isolates with bla(CTX-M-15) were characterized by phylogenetic grouping, PFGE and multilocus sequence typing (MLST). The bla(CTX-M-15) plasmids were analysed by replicon typing, plasmid MLST, S1 nuclease PFGE and Southern blot hybridization experiments.Twenty-one E. coli (livestock, food and a toy; 4.0%) and two Salmonella (horse and swine; 1.3%) isolates were CTX-M-15 producers. E. coli isolates were mainly ascribed to three clonal lineages of sequence types ST678 (German outbreak with enteroaggregative Shiga-toxin-producing E. coli O104:H4; salmon, cucumber and a toy), ST410 (poultry, swine and cattle farms) and ST167/617 (swine farms and turkey meat). The bla(CTX-M-15) genes were located on IncI1 and multireplicon IncF plasmids or on the chromosome of E. coli ST410 isolates.The prevalence of CTX-M-15-producing isolates from non-human sources in Germany is still low. The bla(CTX-M-15) gene is, however, present in multidrug-resistant E. coli clones with pathogenic potential in livestock and food. The maintenance of the bla(CTX-M-15) gene due to chromosomal carriage is noteworthy. The possibility of an exchange of CTX-M-15-producing isolates or plasmids between livestock and humans (in both directions) deserves continuous surveillance.