MEASUREMENT OF EICOSANOIDS IN MENSTRUAL FLUID BY THE COMBINED USE OF HIGH-PRESSURE CHROMATOGRAPHY AND RADIOIMMUNOASSAY
MEASUREMENT OF EICOSANOIDS IN MENSTRUAL FLUID BY THE COMBINED USE OF HIGH-PRESSURE CHROMATOGRAPHY AND RADIOIMMUNOASSAY
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DOI：
10.1016/0090-6980(93)90118-q
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发表时间：
1993-05-01
期刊：
影响因子：
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通讯作者：
JANISCH, H
中科院分区：
文献类型：
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作者：
HOFER, G;BIEGLMAYER, C;JANISCH, H
描述了用于定量通过棉塞收集的经血中的各种类二十烷酸（环氧合酶产物：6-KETO、TXB 2、PGE 2、PGF 2 α、DHK;脂氧合酶产物：5-、12-、15-HETE、LTB 4、LTC 4、LTD 4、LTE 4）的方法。样品用酸化乙醇提取。在通过SEP-PACK C18柱纯化后，使用三元梯度系统通过反相HPLC分离化合物。通过放射免疫测定法测量分级洗脱液的类花生酸浓度，并校正回收率。12-HETE是经血中花生四烯酸最主要的代谢物（平均值：1174纳克/克血液）。除PGF 2 α和TXB 2（平均值：分别为343和212 ng/g血液）外，检测到其他类花生酸的浓度明显较低。由奥地利科学研究基金会（Fonds zur Forderung der wissenschaftlichen Forschung）P7137号资助。
Methods are described for the quantification of various eicosanoids (cyclooxygenase products: 6-KETO, TXB2, PGE2, PGF2alpha, DHK; lipoxygenase products: 5-, 12-, 15-HETE, LTB4, LTC4, LTD4, LTE4) in menstrual blood collected by tampons. Samples were extracted with acidified ethanol. After purification by SEP-PACK C18 columns, the compounds were separated by reversed phase HPLC using a ternary gradient system. The eicosanoid concentrations of the fractionated eluents were measured by radioimmunoassay and corrected for recovery. 12-HETE was the most prominent metabolite of arachidonic acid in menstrual blood (mean: 1174 ng/g blood). With the exception of PGF2alpha and TXB2 (mean: 343 and 212 ng/g blood, respectively) other eicosanoids were detected in remarkable lower concentrations. Supported by grant #P7137 from the Austrian ''Fonds zur Forderung der wissenschaftlichen Forschung''