Nitric oxide and cellular immunity in experimental cutaneous leishmaniasis
Nitric oxide and cellular immunity in experimental cutaneous leishmaniasis
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DOI：
10.1046/j.1365-2230.2003.01206.x
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发表时间：
2003-05-01
影响因子：
4.1
通讯作者：
Tapia, FJ
中科院分区：
文献类型：
--
作者：
Díaz, NL;Fernández, M;Tapia, FJ
我们研究了局部和全身的一氧化氮（NO）的生产和细胞因子的模式在易感BALB/c和耐药C57 BL/6小鼠感染墨西哥利什曼原虫的过程中。检测血清中NO衍生物，并通过免疫组织化学方法检测病变中诱导型一氧化氮合酶（iNOS）、干扰素（IFN-γ）、白细胞介素（IL-4）和表皮朗格汉斯细胞（LC）的表达。循环NO浓度、iNOS+细胞密度、IFN-γ + Th 1细胞和CD 205+朗格汉斯细胞在耐药C57 BL/6小鼠的早期病变中较高。与此相反，易感BALB/c小鼠出现慢性和进行性病变，IL-4+ Th 2细胞占优势。在易感和耐药小鼠中，病变大小和淋巴结体积遵循类似的过程。早期的局部和全身生产的NO在耐药小鼠可能与过早生产的IFN-γ观察，有助于解决病变。
We examined the local and systemic production of nitric oxide (NO) and the pattern of cytokine during the course of Leishmania mexicana infection in susceptible BALB/c and resistant C57BL/6 mice. NO derivatives were measured in serum, and the expression of inducible nitric oxide synthase (iNOS), interferon (IFN-gamma), interleukin (IL-4) and epidermal Langerhans cells (LC) was measured in the lesions by immunohistology. Circulating NO concentrations, iNOS+ cell density, IFN-gamma+ Th1 cells and CD205+ Langerhans cells were higher in early lesions of resistant C57BL/6 mice. In contrast, susceptible BALB/c mice developed chronic and progressive lesions with a predominance of IL-4+ Th2 cells. In both susceptible and resistant mice, lesion size and lymph node volume followed a similar course. The early local and systemic production of NO in resistant mice may be related with the premature production of IFN-gamma observed, contributing to the resolution of the lesion.