Sensitivities of Radioimmunoassay and Enzyme‐Linked Immunosorbent Assay for Detection of Hepatitis B Surface Antigen
Sensitivities of Radioimmunoassay and Enzyme‐Linked Immunosorbent Assay for Detection of Hepatitis B Surface Antigen
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放射免疫分析和酶联免疫吸附分析检测乙型肝炎表面抗原的灵敏度
DOI：
10.1111/j.1423-0410.1979.tb04414.x
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发表时间：
1979
期刊：
影响因子：
2.7
通讯作者：
S. L. Hope
中科院分区：
文献类型：
--
作者：
C. Hyland;E. Mason;P. Harden;A. Shaw;D. Maurer;S. L. Hope
抽象的。发现使用Ausria II（Abbott）的放射免疫测定法（RIA）检测HBsAg的灵敏度高于Hepanostika Micro‐Elisa系统（Organon Teknika），对于亚型“ad”（有一个例外）为5- 20倍，对于亚型“ay”为2- 5倍。还在7个不同实验室提供的178份选定血清样本的筛选中评价了RIA和酶联免疫吸附试验（Elisa）的灵敏度。其中RIA阳性149例，Elisa阳性142例，CEP阳性36例。
Abstract. Radioimmunossay (RIA) using Ausria II (Abbott) was found to be more sensitive for the detection of HBsAg than the Hepanostika Micro‐Elisa system (Organon Teknika), by a factor of 5– to 20–fold for subtype ‘ad’ (with one exception), and 2– to 5–fold for subtype ‘ay’. The sensitivities of RIA and the enzyme‐linked immunosorbent assay (Elisa) have also been evaluated in the screening of 178 selected serum samples contributed by 7 different laboratories. Of these, 149 were found to be positive for HBsAg by RIA, 142 were positive by Elisa and 36 were positive by CEP.