Transcriptomic Analysis of the Spider Venom Gland Reveals Venom Diversity and Species Consanguinity
Transcriptomic Analysis of the Spider Venom Gland Reveals Venom Diversity and Species Consanguinity
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蜘蛛毒腺的转录组分析揭示了毒液多样性和物种血缘关系
DOI：
10.3390/toxins11020068
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发表时间：
2019-02-01
期刊：
影响因子：
4.2
通讯作者：
Liu, Zhonghua
中科院分区：
文献类型：
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作者：
Hu, Zhaotun;Chen, Bo;Liu, Zhonghua
嘉福月衣（S. jiafu）是中国蜘蛛的一个新种。它生活在同一个栖息地的各种其他有毒蜘蛛，包括Chilobrachys jingzhao（C。jingzhao）、虎纹捕鸟蛛Selenocosmia huwena（S. huwena）和Macrothele raveni（M. raveni）。这些不同种类蜘蛛的毒液在生化和电生理特性方面表现出一些相似性和一些差异。为了阐明毒肽毒素的多样性并建立不同蜘蛛物种之间的进化关系，我们首先对来自S.家福。我们鉴定了146个新的毒素样序列，它们被分为18个不同的超家族。然后将该转录组与C. Jingzhao的研究表明，这两种蜘蛛毒液的假定毒素可能起源于同一祖先，尽管新的毒素在这两个物种中独立进化。BLAST检索和药理学分析显示，这两种毒液具有相似的钠通道调节活性。这项研究提供了对两种密切相关的蜘蛛毒液的了解，这将有助于了解它们毒素的结构和功能。
Selenocosmia jiafu (S. jiafu) has been recently identified as a new species of spider in China. It lives in the same habitat as various other venomous spiders, including Chilobrachys jingzhao (C. jingzhao), Selenocosmia huwena (S. huwena), and Macrothele raveni (M. raveni). The venom from these different species of spiders exhibits some similarities and some differences in terms of their biochemical and electrophysiological properties. With the objective to illustrate the diversity in venom peptide toxins and to establish the evolutionary relationship between different spider species, we first performed transcriptomic analysis on a cDNA library from the venom gland of S. jiafu. We identified 146 novel toxin-like sequences, which were classified into eighteen different superfamilies. This transcriptome was then compared with that of C. jingzhao, which revealed that the putative toxins from both spider venoms may have originated from the same ancestor, although novel toxins evolved independently in the two species. A BLAST search and pharmacological analysis revealed that the two venoms have similar sodium channel modulation activity. This study provides insights into the venom of two closely related species of spider, which will prove useful towards understanding the structure and function of their toxins.