Cytogenetic analysis of human acute and chronic myeloid leukemic cells cloned in agar culture
Cytogenetic analysis of human acute and chronic myeloid leukemic cells cloned in agar culture
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琼脂培养中克隆的人急慢性粒细胞白血病细胞的细胞遗传学分析
DOI：
10.1002/ijc.2910110117
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发表时间：
1973
影响因子：
6.4
通讯作者：
D. Metcalf
中科院分区：
文献类型：
--
作者：
M. Moore;D. Metcalf
对12例有核型标记的急性或慢性髓系或髓单细胞白血病患者的血液或骨髓琼脂培养进行了核型分析。在培养中形成的粒细胞集落和团簇显示来自白血病群体的代表性细胞。在两例缓解期急性白血病患者中，骨髓细胞培养出正常核型的正常菌落，但在两例缓解期慢性髓系白血病患者中，菌落细胞中Ph1异常持续存在。琼脂培养技术似乎是理想的出现和消失的白血病和正常的粒细胞生成群体的患者这些类型的白血病。
Karyotypic analysis was performed on agar cultures of blood or bone marrow from 12 patients with acute or chronic myeloid or myelomonocytic leukemia in whom karyotypic markers were present. The granulocytic colonies and clusters which developed on culture were shown to be derived from representative cells of the leukemic population. In two patients with acute leukemia in remission, normal colonies with a normal karyotype were grown from marrow cells but in two patients with chronic myeloid leukemia in remission the Ph1 abnormality persisted in colony cells. The agar culture technique appears to be ideal for following the emergence and disappearance of leukemic and normal granulopoietic populations in patients with these types of leukemia.