Effects of maternal diabetes on early embryogenesis: II. Hyperglycemia-induced exencephaly.
Effects of maternal diabetes on early embryogenesis: II. Hyperglycemia-induced exencephaly.
复制标题
母亲糖尿病对早期胚胎发生的影响：II。
DOI：
10.1002/tera.1420210311
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Sadler,TW
中科院分区：
文献类型：
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作者：
Sadler,TW
采用全胚胎培养技术研究了高血糖对胚胎发育早期的致畸作用。将年轻（0-1和2-3体节）和老年（4-6体节）小鼠胚胎暴露于相当于正常血糖水平5倍和8倍的过量葡萄糖的糖尿病水平24小时。在任一浓度下保存的胚胎中观察到神经管缺陷（露脑畸形）的发生率较高，且该效应具有剂量和年龄依赖性。因此，较年轻的胚胎（0-3体节）比较老的胚胎更容易受到影响，并且在较高剂量下受到影响的胚胎比较低剂量下更多。虽然观察到畸形，但对照和处理培养物之间的总蛋白量相似，但最年轻（0-1体节）胚胎除外，其蛋白量显著低于对照。培养开始后，对在8×葡萄糖中维持4、8和24 h的胚胎进行组织学分析，结果显示神经上皮中仅存在少量固缩碎片，未发现其他异常原因。本研究中观察到的神经管闭合抑制与糖尿病母亲的婴儿中偶尔描述的相似，并表明高血糖可能在畸形的产生中发挥作用。
Teratogenic effects of hyperglycemia on early stages of embryogenesis were investigated using the whole embryo culture technique. Young (0–1 and 2–3 somites) and old (4–6 somites) mouse embryos were exposed for 24 h to diabetic levels of excess glucose equal to 5× and 8× the normal blood glucose levels. A high frequency of neural tube defects (exencephaly) was observed in embryos maintained at either concentration, and this effect was dose‐ and age‐dependent. Thus, younger embryos (0–3 somites) were more frequently affected than older ones, and more embryos were affected at the higher dose than the lower one. Although malformations were observed, amounts of total protein were similar between control and treated cultures except for the youngest (0–1 somite) embryos, which had significantly lower amounts of protein than controls. Histological analysis of embryos maintained in 8× glucose for 4, 8, and 24 h after initiation of culture showed only a slight amount of pyknotic debris in the neuroepithelium and revealed no other causes for the abnormality. Inhibition of neural tube closure observed in this study is similar to that occasionally described in infants of diabetic mothers, and suggests that hyperglycemia may play a role in production of the malformation.