STUDIES ON PRODUCTION OF PERITHECIAL STROMATA BY CORDYCEPS MILITARIS IN ARTIFICIAL CULTURE
STUDIES ON PRODUCTION OF PERITHECIAL STROMATA BY CORDYCEPS MILITARIS IN ARTIFICIAL CULTURE
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DOI：
10.1139/b68-071
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发表时间：
1968-01-01
期刊：
影响因子：
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通讯作者：
MADELIN, MF
中科院分区：
文献类型：
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作者：
BASITH, M;MADELIN, MF
对人工培养中影响蛹虫草子囊座座产生和成熟的一些营养和环境因素进行了研究。该真菌在含有多种碳源和氮源的确定培养基中无性生长，不添加维生素，并且可以使用硝酸盐作为唯一氮源，但仅在特殊营养条件下形成子座，例如由灭菌浸泡的米粒培养基提供的营养条件。子座的启动需要超过 3 英尺烛光的照明，而成熟子囊壳的产生需要强度大于 15 至 90 ft-c 之间的阈值。高于约 22 °C 的温度会抑制基质发育。过度通气也是如此，它会去除培养物产生的气态或挥发性刺激物质。发现适合基质生产的培养基包括含有与血红蛋白或酪蛋白或蛋白胨相关的淀粉或蔗糖的矿物盐溶液。单独或混合使用的氨基酸和葡萄糖均不受欢迎。没有证据表明结果需要生长因子。结果支持这样的观点，即含有碳和氮源的培养基有利于基质生产，这些碳源和氮源在水解之前不能被同化，因此可以长期维持低浓度的可同化营养物质。
Some nutritional and environmental factors which influence the production and maturation of perithecial stromata ofCordyceps militaris(Linnaeus) Link in artificial culture have been studied. The fungus grows vegetatively in defined media with a variety of carbon and nitrogen sources without added vitamins, and can use nitrate as sole nitrogen source, but forms stromata only under special nutritional conditions such as are provided by a medium of sterilized soaked rice grains. Illumination at more than 3 foot-candles is necessary for stromata to be initiated, and an intensity greater than a threshold value between 15 and 90 ft-c is necessary for production of mature perithecia. Temperatures above about 22 °C depress stroma development. So, too, does excessive aeration, which removes a gaseous or volatile stimulatory substance which is produced by the cultures. Media found suitable for stroma production include mineral salt solutions with either starch or sucrose associated with either haemoglobin or casein or peptone. Amino acids, used singly or in mixture, and glucose were not favorable. There was no evidence that fruiting required growth factors. The results support the view that stroma production is favored by media with carbon and nitrogen sources which cannot be assimilated until they have been hydrolyzed, and which consequently sustain favorably low concentrations of assimilable nutrients over a long period.