The interaction of concanavalin A with blood-group-substance glycoproteins from human secretions.
The interaction of concanavalin A with blood-group-substance glycoproteins from human secretions.
复制标题
刀豆球蛋白 A 与人类分泌物中的血型物质糖蛋白的相互作用。
作者：
A. Clarke;M. Denborough
1.将胃液、唾液和卵巢囊液在氯化铯密度梯度中离心至平衡，得到糖蛋白组分。2.两名O组非分泌者、两名O组分泌者和三名A组分泌者的胃液糖蛋白组分均与刀豆蛋白A形成不溶性的络合物。3.血凝抑制法测定，与刀豆蛋白A相互作用最大的组份具有最大的血型活性。胃糖蛋白的硫酸盐含量与刀豆蛋白A的相互作用能力无关。4.刀豆蛋白A与唾液或卵巢囊液中的糖蛋白未发生相互作用，提示胃液中的血型物质糖蛋白与唾液和卵巢囊液中的糖蛋白有结构上的差异。5.每种分泌物，胃液、唾液和卵巢囊液的蛋白质组分均与刀豆蛋白A相互作用。
1. Gastric juice, saliva and ovarian-cyst fluid were fractionated into glycoprotein components by centrifuging to equilibrium in a caesium chloride density gradient. 2. The glycoprotein fractions from the gastric juice of two group O non-secretors, two group O secretors and three group A secretors all formed insoluble complexes with concanavalin A. 3. Fractions showing maximum interaction with concanavalin A had maximum blood-group activity measured by the haemagglutination-inhibition technique. The sulphate content of the gastric glycoproteins was unrelated to the capacity to interact with concanavalin A. 4. No interaction was found between concanavalin A and the glycoprotein fractions from any of the saliva or ovarian-cyst-fluid samples tested, implying that there is a structural difference in blood-group-substance glycoproteins in gastric juice when compared with those in saliva and ovarian-cyst fluid. 5. The protein components of each of the secretions tested, gastric juice, saliva and ovarian-cyst fluid, interacted with concanavalin A.