Methicillin-resistant Staphylococcus aureus (MRSA) in slaughtered pigs and abattoir workers in Italy
Methicillin-resistant Staphylococcus aureus (MRSA) in slaughtered pigs and abattoir workers in Italy
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DOI：
10.1016/j.fm.2015.04.007
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发表时间：
2015-10-01
影响因子：
5.3
通讯作者：
Parisi, Antonio
中科院分区：
文献类型：
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作者：
Normanno, Giovanni;Dambrosio, Angela;Parisi, Antonio
耐甲氧西林金黄色葡萄球菌 (MRSA) 是存在于医院环境 (HA-MRSA)、社区 (CA-MRSA) 和包括猪在内的牲畜 (LA-MRSA) 中的病原体。 MRSA 可能在屠宰过程中进入人类食物链，并可能感染直接接触猪或猪肉产品的人类。本研究旨在确定从意大利南部工业屠宰场的猪和工人中分离出的 MRSA 的流行情况和特征。使用 PCR 对总共 215 份猪鼻拭子进行了 MRSA 筛查。从每个 mecA/nuc PCR 阳性样本中检测到 MRSA 分离株，并通过 spa 分型、多位点序列分型、SCC-mec 和 PantonValentine 杀白细胞素 (PVL) 进行表征，还测试了葡萄球菌肠毒素 (SE) 的产生。从 215 只猪鼻拭子中获得了 81 个 MRSA 分离株 (37.6%)；对其中 37 种分离株进行了进一步表征，并显示出 18 种不同的 spa 类型和 8 种不同的 ST。最常恢复的 ST 是 ST398（CC398-t034、t011、t899、t1939 - 43.2%），其次是 ST8（CC8-t008、t064、t2953、t5270 - 24.3%）和 ST1（CC1-t127、t174、t2207 - 10.8%)。从 113 份人类拭子中分离出 9 株 MRSA；分离株显示出 5 种不同的 spa 型和 5 种不同的 ST，包括新型 ST2794 (t159)。恢复的最具代表性的 ST 是 ST1 (CC1-t127) 和 ST398 (CC398-t034) (33.3%)。没有一种 MRSA 分离株表现出产生 SE 和 PVL 的能力，并且所有菌株都对两类或更多类抗菌药物产生耐药性。这项研究显示了意大利屠宰猪和屠宰场员工中 MRSA 菌株的巨大遗传多样性，并清楚地表明需要提高卫生标准，以降低与处理/消费含有 MRSA 的生猪肉相关的职业和食源性感染风险。 (C) 2015 Elsevier Ltd. 保留所有权利。
Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogen present in the hospital environment (HA-MRSA), in the community (CA-MRSA) and in livestock, including pigs (LA-MRSA). MRSA may enter the human food chain during slaughtering and may infect humans coming into direct contact with pigs or pork products. This study aimed to determine the prevalence and characteristics of MRSA isolated from pigs and workers at industrial abattoirs in southern Italy. A total of 215 pig nasal swabs were screened for the presence of MRSA using PCR. An MRSA isolate was detected from each mecA/nuc PCR-positive sample and characterized by spa-typing, Multi-Locus Sequence Typing, SCC-mec and PantonValentine Leukocidin (PVL), and also tested for the production of staphylococcal enterotoxins (SEs).Eighty-one MRSA isolates (37.6%) were obtained from the 215 pig nasal swabs; 37 of these isolates were further characterized, and showed 18 different spa-types and 8 different STs. The most frequently recovered STs were ST398 (CC398-t034, t011, t899, t1939 - 43.2%) followed by ST8 (CC8-t008, t064, t2953, t5270 - 24.3%) and ST1 (CC1-t127, t174, t2207 - 10.8%).Nine MRSA isolates were obtained from the 113 human swabs; the isolates showed 5 different spatypes and 5 different STs, including the novel ST2794 (t159). The most representative STs recovered were ST1 (CC1-t127) and ST398 (CC398-t034) (33.3%). None of the MRSA isolates showed the ability to produce SEs and PVL and all resulted resistant to two or more classes of antimicrobials. This study shows the great genetic diversity of MRSA strains in slaughtered pigs and in abattoir employees in Italy, and clearly demonstrates the need for improved hygiene standards to reduce the risk of occupational and foodborne infection linked to the handling/consumption of raw pork containing MRSA. (C) 2015 Elsevier Ltd. All rights reserved.