PHENOTYPIC ANALYSIS OF SYNOVIAL TISSUE AND PERIPHERAL-BLOOD LYMPHOCYTES ISOLATED FROM PATIENTS WITH RHEUMATOID-ARTHRITIS
PHENOTYPIC ANALYSIS OF SYNOVIAL TISSUE AND PERIPHERAL-BLOOD LYMPHOCYTES ISOLATED FROM PATIENTS WITH RHEUMATOID-ARTHRITIS
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DOI：
10.1002/art.1780311003
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发表时间：
1988-10-01
影响因子：
--
通讯作者：
LIPSKY, PE
中科院分区：
文献类型：
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作者：
CUSH, JJ;LIPSKY, PE
应用荧光细胞分析法对7例类风湿关节炎（RA）患者新鲜分离的滑膜组织淋巴细胞（STL）和外周血淋巴细胞（PBL）的表面表型和活化状态进行了分析。将切除的滑膜酶消化以获得组织来源的淋巴细胞。用各种单克隆抗体对患者PBL和STL进行间接免疫荧光染色，未能揭示CD 4+（辅助/诱导）PBL或STL数量的一致变化。而类风湿性STL患者CD 8+（抑制/细胞毒）细胞数量明显减少（P < 0.05）。RA PBL和STL中T细胞分化抗原CD 3和CD 4的密度与对照PBL相比显著降低。当正常PBL进行相同的酶消化时，未观察到抗原密度的这些差异。RA PBL和STL均表现出HLA-DR抗原表达增加，而白细胞介素-2受体表达无增加。患者PBL的T细胞分化抗原和活化抗原表达的改变与STL中观察到的异常密切相关。相反，RA患者的STL显示粘附相关糖蛋白（白细胞功能相关1 [LFA-1]和极晚期活化1 [VLA-1]抗原）表达增加，而自体PBL未观察到。这些研究表明，从RA患者的滑膜组织中分离的淋巴细胞具有活化的表型，例如由CD 3和CD 4的调节和HLA-DR的表达。STL的粘附分子LFA-1和VLA-1的表达增强可能在这些细胞的局部炎症滑膜中发挥作用。
Cytofluorometric analysis was performed to characterize the surface phenotype and activation status of freshly isolated synovial tissues lymphocytes (STL) and peripheral blood lymphocytes (PBL) from 7 patients with rheumatoid arthritis (RA). Proliferative synovium was enzymatically digested to obtain tissue-derived lymphocytes. Indirect immunofluorescent staining of patient PBL and STL with a variety of monoclonal antibodies failed to reveal a consistent alteration in the number of CD4+ (helper/inducer) PBL or STL. However, there was a significant decrease in the number of CD8+ (suppressor/cytotoxic) cells in rheumatoid STL (P < 0.05). A significant reduction in the density of the T cell differentiation antigens CD3 and CD4 was observed in RA PBL and STL, compared with control PBL. These differences in antigen density were not seen when normal PBL were subjected to the same enzymatic digestion. Both RA PBL and STL manifested increased expression of HLA-DR antigens, without augmentation of interleukin-2 receptor expression. Alterations in the expression of the T cell differentiation antigens and activation antigens by patient PBL closely paralleled the abnormalities observed in STL. In contrast, STL of patients with RA exhibited an increase in the expression of the adhesion-related glycoproteins (leukocytes function-associated 1 [LFA-1] and very late activation 1 [VLA-1] antigens), not observed with autologous PBL. These studies demonstrate that lymphocytes isolated from the synovial tissue of RA patients bear an activated phenotype, exemplified by the modulation of CD3 and CD4 and the expression of HLA-DR. The enhanced expression of the adhesion molecules LFA-1 and VLA-1 by STL may play a role in the localization of these cells to the inflamed synovium.