Expression of IL-10 and TNF-α in Rats with Cerebral Infarction after Transplantation with Mesenchymal Stem Cells
Expression of IL-10 and TNF-α in Rats with Cerebral Infarction after Transplantation with Mesenchymal Stem Cells
复制标题
DOI：
10.1038/cmi.2009.28
复制
发表时间：
2009-06-01
影响因子：
24.1
通讯作者：
Wen, Jinsen
中科院分区：
文献类型：
--
作者：
Liu, Nan;Chen, Ronghua;Wen, Jinsen
我们研究了骨髓间充质干细胞（MSCs）移植对大鼠MCAO（大脑中动脉闭塞）模型神经功能恢复的影响及其机制。从雄性 Sprague Dawley (SD) 大鼠的骨髓中分离出 MSC。雌性成年SD大鼠被随机分为4组：假手术组、MCAO组、载体组和MCAO+MSC治疗组。 MSCs治疗组大鼠侧脑室注射MSCs，媒介物组给予等体积的PBS。 MCAO后第1天和第4天通过RT-PCR和ELISA检测IL-10和TNF-α的表达。通过TTC染色测量梗塞体积。所有大鼠在 MCAO 之前以及之后 1、4 和 14 天均接受行为测试。移植后第 14 天，与对照组相比，MSC 显着改善了功能恢复。与MCAO组和vehicle组相比，MSCs组IL-10 mRNA表达及其蛋白水平显着上调。然而，与MCAO组和媒介物组相比，MSCs组在MCAO后第4天TNF-α的表达显着降低。结果，MSC 移植在第 1 天和第 4 天显着减少了梗塞体积。这项研究强烈表明，MSC 移植可以部分通过调节大脑中 IL-10 和 TNF-α 的表达来减少大鼠局灶性脑缺血后的神经元损伤。细胞和分子免疫学。 2009；6(3)：207-213。
We investigated the effects of bone marrow-derived mesenchymal stem cells (MSCs) transplantation on the recovery of neurological functions in rat's MCAO (middle cerebral artery occlusion) model and its mechanism. MSCs were isolated from bone marrow of male Sprague Dawley (SD) rats. Female adult SD rats were randomly assigned into 4 groups: sham-operated group, MCAO group, vehicle group and MCAO + MSCs-treated group. MSCs were injected into the lateral ventricle of rats in the MSCs-treated group and the same volume of PBS was given to the vehicle group. The expressions of IL-10 and TNF-alpha were assayed by RT-PCR and ELISA detections at day 1 and 4 after MCAO. The infarction volume was measured by TTC-staining. All rats underwent behavioral tests before, as well as 1, 4, and 14 days after MCAO. MSCs significantly improved functional recovery compared with the control at day 14 after transplantation. Compared with the MCAO group and the vehicle group, the expression of IL-10 mRNA and its protein level in the MSCs group significantly upregulated. However, the expression of TNF-alpha at day 4 after MCAO in the MSCs group significantly decreased compared with that of the MCAO group and the vehicle group. As a result, transplantation with MSCs significantly decreased infarct volume at day 1 and 4. This study strongly suggested transplantation with MSCs could reduce neuronal injury post focal cerebral ischemia in rats partly by regulating the expressions of IL-10 and TNF-alpha in the brain. Cellular & Molecular Immunology. 2009;6(3):207-213.