Virus-specific spatial differences in the interference with silencing of the chs-A gene in non-transgenic petunia.
Virus-specific spatial differences in the interference with silencing of the chs-A gene in non-transgenic petunia.
复制标题
非转基因矮牵牛中病毒特异性干扰 chs-A 基因沉默的空间差异。
DOI：
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发表时间：
2001
影响因子：
3.8
通讯作者：
M. Tepfer
中科院分区：
文献类型：
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作者：
P. Teycheney;M. Tepfer
马铃薯Y病毒和烟草蚀刻病毒以及黄瓜花叶黄瓜病毒等马铃薯病毒会干扰转录后基因沉默（PTGS）。当红星型矮牵牛（其花有白色和色素相间的部分）被这些病毒感染时，每种病毒诱导了不同模式的花花青素色素沉着的恢复。通过干扰查尔酮合成酶A （chs-A）基因的PTGS发生的白色扇区彩色表型的局部逆转与chs-A mRNA和病毒浓度的局部升高有关。我们的研究结果表明，病毒感染可以干扰原生植物基因的PTGS，这可能对症状表达产生深远的影响。
Potyviruses, such as potato virus Y and tobacco etch virus, as well as cucumber mosaic cucumovirus, interfere with post-transcriptional gene silencing (PTGS). When RedStar-type Petunia hybrida cultivars, whose flowers have alternating white and pigmented sectors, were infected with these viruses, each virus induced a different pattern of restoration of floral anthocyanin pigmentation. Local reversion to coloured phenotypes in the white sectors, which occurred through interference with PTGS of the chalcone synthase A (chs-A) gene, was correlated with locally increased levels of chs-A mRNA and virus concentration. Our results show that virus infection can interfere with PTGS of a native plant gene, and that this can have profound effects on symptom expression.