F-Specific RNA Bacteriophages as Model Viruses in UV Disinfection of Wastewater
F-Specific RNA Bacteriophages as Model Viruses in UV Disinfection of Wastewater
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F 特异性 RNA 噬菌体作为废水紫外线消毒模型病毒
DOI：
10.2166/wst.1991.0088
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
M. V. Olphen
中科院分区：
文献类型：
--
作者：
A. Havelaar;T. Nieuwstad;C. Meulemans;M. V. Olphen
F-特异性RNA(FRNA)噬菌体可以用简单、快速的方法检测废水中的F-特异性RNA噬菌体。它们在紫外光照射下的灭活符合一级动力学，相对简单的公式可以描述废水对紫外光的吸收效果和多色灯的波长相关杀灭效率。因为微生物可以在高浓度下生长，所以全规模反应器的生物校准只需要相对少量的噬菌体培养。自然产生的噬菌体对紫外线的抵抗力强于体细胞噬菌体、大肠杆菌和粪链球菌。灭活速率常数与呼肠孤病毒几乎相同，表明噬菌体作为紫外线灭活病毒的过程指示剂是合适的。MS2纯培养的失活速度几乎是自然产生的噬菌体的两倍，这表明有必要根据现场数据而不是实验室实验来设计实际应用的反应堆。
F-specific RNA (FRNA) bacteriophages can be assayed in wastewater by simple and rapid methods. Their inactivation by UV radiation follows first-order kinetics and relatively simple formulas can describe the effect of UV absorbance by wastewater and the wavelength-dependent killing efficiency of polychromatic lamps. Because the organisms can be grown in high concentrations, biological calibration of full-scale reactors requires only relatively small volumes of phage culture. Naturally occurring FRNA-phages were more resistant to UV than somatic coliphages, Escherichia coli and faecal streptococci. The inactivation rate constant was almost equal to that of reoviruses, which underlines the suitability of FRNA-phages as a process indicator for UV inactivation of viruses. A pure culture of MS2 was inactivated at a rate which was almost twice that of naturally occurring FRNA-phages, indicating the necessity of designing reactors for practical applications on field-data rather than laboratory experiments.