Structure of an interferon-α2 gene expressed in the bovine conceptus early in gestation
Structure of an interferon-α2 gene expressed in the bovine conceptus early in gestation
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妊娠早期牛胚胎中表达的干扰素-α2基因的结构
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
A. Flint
中科院分区：
文献类型：
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作者：
H. J. Stewart;S. McCann;A. Flint
用绵羊滋养层干扰素（IFN）cDNA探针分别从牛基因组文库和牛胚胎（交配后第18天）cDNA文库中分离得到了牛滋养层干扰素（IFN）的基因组和cDNA克隆。这两个DNA序列是相同的;序列分析表明，牛滋养层IFN和绵羊滋养层IFN的氨基酸序列之间的80%的同一性，和70%的同一性与以前确定的牛IFN-α 2。牛基因组DNA的Southern印迹法表明存在至少三个滋养层IFN基因。引物延伸分析确定了牛IFN基因5'侧翼区的转录起始位点。计算机辅助分析的5'侧翼序列表明，与牛IFN-α 2的相似性和可能的病毒诱导序列的存在。
: Genomic and cDNA clones for bovine trophoblast interferon (IFN) have been isolated by probing a bovine genomic library and a bovine embryonic (day-18 post coitus) cDNA library respectively with the ovine trophoblast IFN cDNA. The two DNA sequences were identical; sequence analysis demonstrated 80% identity between the amino acid sequence of bovine trophoblast IFN and ovine trophoblast IFN, and 70% identity with a previously identified bovine IFN-alpha 2. Southern blotting of bovine genomic DNA indicated the presence of a minimum of three trophoblast IFN genes. Primer extension analysis identified the transcription start site in the 5' flanking region of the bovine IFN gene. Computer-aided analysis of the 5' flanking sequence demonstrated a similarity with that of bovine IFN-alpha 2 and the existence of a possible viral induction sequence.